凋亡抑制基因Bcl-2和增殖细胞核抗原在乳腺导管浸润癌中的表达及意义

目的:探讨凋亡抑制基因Bcl-2和增殖细胞核抗原(PCNA)在乳腺导管浸润癌中的表达及与各临床参数的关系.方法:免疫组织化学染色法对35例乳腺导管浸润癌患者进行检测.结果:Bcl-2和PCNA蛋白表达均为68.6%;Bcl-2表达与癌组织分级及有无淋巴结转移呈负相关(P＜0.01),与雌激素(ER)水平及5年生存期呈正相关(P＜0.01),与肿物大小无关;PCNA表达与癌组织分级及肿物大小呈正相关(P＜0.05),与ER水平及5年生存期呈负相关(P＜0.05),与淋巴结有无转移无关;Bcl-2与PCNA表达呈负相关.结论:Bcl-2高表达、ER水平高、PCNA表达低者生存期长、预后好,Bcl-2和PCNA可作为判定乳腺导管浸润癌恶性程度及预后的有效指标.

Expressions of Bcl-2 and PCNA in invasive ductal carcinoma of breast and their clinical significance

Objective:To examine the expressions of apoptosis suppressor gene Bcl 2 and proliferating cell nuclear antigen (PCNA) in invasive ductal carcinoma of breast and their clinical significance. Methods:35 cases of paraffin embedded specimens of invasive ductal breast cercinoma were used for immunohistochemical study.Results:The positive rates of both Bcl 2 and PCNA expression were 68.6%.The expression rate of Bcl 2 was closely correlated with the level of estrogen receptor (ER) and the five year survival ( P <0.01,respectively) whereas the expression of Bcl 2 was not related to tumor size.The relationship between the expression of PCNA and histological grade or lymphnode metastases was negative correalted ( P <0.05).The expression of PCNA was closely correlated with tumor size and histological grade ( P <0.05),negative correlated with the age,the level of estrogenrceptor and the survival duration ( P <0.05),no related to lymph node netastases.Conclusion:The results suggest that either over expression of Bcl 2 and or low expression of PCNA predicts a good prognosis and long survial duration.