真核表达载体pcDNA3-FHIT的构建及其在COS-1细胞中瞬时表达

目的 :构建FHIT基因的真核表达载体及测定其在COS 1细胞中的瞬时表达。方法 :应用RT PCR方法从正常人甲状腺组织中克隆出FHIT基因 ,在KpnⅠ和 BstXⅠ两个酶切位点插入真核表达载体 pcDNA3中 ,通过酶切分析、基因测序和免疫细胞化学方法鉴定插入基因片段的正确性。结果 :FHIT基因在载体 pcDNA3中插入的位点是正确的 ,没有缺失、插入等突变 ,并在COS 1细胞中有较高的瞬时表达。结论 :成功构建了FHIT基因的真核表达载体 ,并获得其在COS 1细胞中较高的瞬时表达 ,为该基因在基因治疗中的研究提供了有力的分子工具

Construction of Eukaryotic Expression Plasmid pcDNA3-FHIT and Its Transient Expression in COS-1 Cells

Objective: To construct eukaryotic expression plasmid pcDNA3-fragile histidine triad(FHIT)and obtain its transient expression in COS-1 cells. Methods: FHIT gene was cloned from normal human thyroid tissue by RT-PCR and then inserted into eukaryotic expression vector pcDNA3. After the sequence was confirmed, the recombinant plasmid pcDNA3-FHIT was transfected into COS-1 cells by cation liposome. The transient expression in the cells was measured by immunocytochemistry. Results: The sequence of FHIT in pcDNA3 was correct and high expression was obtained in COS-1 cells. Conclusion: The eukaryotic expression plasmid pcDNA3-FHIT was constructed successfully and could highly express FHIT protein in COS-1 cells. This will be potentially useful for the research on gene therapy.