Drug resistance in the fission yeast Schizosaccharomyces pombe: pleiotropic mutations affecting the oleic acid and sterol composition of cell membranes |
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| Authors: | Paul A Johnston Alan Coddington |
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| Institution: | (1) School of Biological Sciences, University of East Anglia, NR4 7TJ Norwich, UK;(2) Present address: Department of Pharmacology, School of Medicine, University of North Carolina, 27514 Chapel Hill, NC, USA |
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| Abstract: | Summary The whole cell lipid and sterol content of the drug resistant strains cyh1, cyh3 and cyh4 was compared with that of wild type by thin layer and gas liquid chromatography and by UV spectrophotometric analysis. The cyh3 and cyh4 strains had a decreased content of the unsaturated 18:1 fatty acid oleic acid, a decreased content of ergosterol and an increased content of 24,28 dehydroergosterol with respect to wild type. The cyh1 strain, however, only showed a decreased content of ergosterol and an increased content of 24,28 dehydro-ergosterol when compared to wild type. |
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| Keywords: | Fission yeast Drug resistance Lipid and sterol composition Membranes |
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