原位肝移植大鼠肝内门-体分流量的变化

目的 测定原位肝移植大鼠移植肝肝内门-体分流量的变化。方法健康清洁级封闭群雄性SD大鼠，分为供体组和受体组，采用简化二袖套法建立大鼠原位肝移植模型（rat orthotopic liver transplantation model。ROLTM）24只，分为移植后2h、4d、7d三组，每组8只。各组于相应时间点进行肝脏活体在体灌注。经小隐静脉插管连续灌注D-山梨醇，同时取门静脉和肝静脉血，用酶分光光度法测定山梨醇浓度，计算肝内门-体分流量。结果2h、4d、7d组肝内门-体分流量分别为39．9％，52．3％，27．6％，组间差异显著，且均比正常大鼠明显增高。结论 ROLTM大鼠移植肝血供恢复后即存在肝内门-体分流，并随时间推移分流量变化呈先增加后减少的过程。

Intrahepatic portal systemic shunting in rats after orthotopic liver transplantation

Objective To determine changes in intrahepatic portal systemic shunting(IPSS).Methods A rat model of orthotopic liver transplantation(ROLTM)was established by using two-cuff technique,and 48 male SD rats were divided into donor-group and acceptor-group.Rats were divided into 3 groups with each group having 8 rats.Rats in group I,II and III received liver reperfusion 2 hours,4 days and 7 days after successful transplantation,respectively.Liver perfusion was performed in vivo through saphenous vein.Intrahepatic portal systemic shunting was determined by calculating the concentration of D-sorbitol in the venous blood samples by using enzymatic spectrophotometric method.Results Intrahepatic portal systemic shunting in 2 h,4 d and 7 d group were 39.9%,52.3% and 27.6%,respectively.Significant differences were found between above 3 groups in intrahepatic shunting that was much higher than that in normal rats.Conclusion Intrahepatic portal systemic shunting in transplanted livers occurred immediately after recovery of blood-supply,and presented changes where intrahepatic shunting increased in early stage but decreased later on.