| 羊膜对YAC-1细胞增殖影响的实验研究 |
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| 引用本文： | 沈玺,叶纹,钟一声. 羊膜对YAC-1细胞增殖影响的实验研究[J]. 眼科新进展, 2000, 20(5): 317-318 |
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| 作者姓名： | 沈玺 叶纹 钟一声 |
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| 作者单位： | 200025 上海市，上海第二医科大学附属瑞金医院眼科 |
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| 基金项目： | 上海市科学技术发展基金重大资助项目(编号：994119008) |
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| 摘 要： | 目的 观察羊膜对其基质面培养的ＹＡＣ－１细胞增殖的影响。方法 将ＹＡＣ－１细胞接种于羊膜基质面，用ＭＴＴ自动比色法分别于接种后１、３、７ｄ检测羊膜对ＹＡＣ－１细胞增殖的影响。结果 接种后１、３、７ｄ，羊膜均对ＹＡＣ－１细胞有明显的促增殖作用（Ｐ〈０．０１）。结论 羊膜有促进肿瘤细胞增殖的艇，对眼表面肿瘤摘除同时行羊膜移植术时需慎重。
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| 关 键 词： | 羊膜 淋巴瘤细胞 细胞增殖 眼表面肿瘤 |
| 文章编号： | 1003-5141(2000)05-0317-02 |
| 修稿时间： | 2000-05-08 |
Effect of amniotic membrane on the proliferation of YAC-1 |
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| SHEN Xi,YE Wen,ZHONG Yi-Sheng. Effect of amniotic membrane on the proliferation of YAC-1[J]. Recent Advances in Ophthalmology, 2000, 20(5): 317-318 |
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| Authors: | SHEN Xi YE Wen ZHONG Yi-Sheng |
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| Abstract: | Objective To investigate the effect of amniotic membrane on the proliferation of YAC 1 in vitro. Methods The YAC 1 cells were cultured with amniotic membrane and their proliferation were observed by MTT automated colorimetry on the lst day, the 3rd day and the 7th day respectively.Results Amniotic membrane could significantly enhance the proliferation of YAC 1 on the lst day, the 3rd day and the 7th day respectively after having been seeded ( P <0 01).Conclusion Amniotic membrane can enhance the proliferation of tumor cells (YAC 1), which should be paid attention to during amniotic membrane transplantation for treating ocular surface lesion caused by epibulbar tumors. |
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| Keywords: | amniotic membrane YAC-1 cell cell proliferation epibulbar tumor |
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