腺病毒介导NDRG2在体外对人食管鳞癌细胞系Eca-109增殖和凋亡的影响

目的：研究抑癌候选基因NDRG2在体外对人食管鳞癌细胞系Eca-109增殖和凋亡的影响。方法：腺病毒介导NDRG2转染人食管鳞癌细胞系Eca-109,逆转录酶-多聚酶链反应（RT-PCR）和Western blot法分别检测NDRG2基因的mRNA和蛋白表达水平,甲基噻唑基四唑法（MTT法）绘制细胞生长曲线,流式细胞仪（FCM）分别分析细胞周期和细胞凋亡。结果：腺病毒介导NDRG2转染人食管鳞癌细胞系Eca-109后,NDRG2的mRNA和蛋白表达水平明显上调,细胞增殖受到明显抑制（P〈0.05）;流式细胞仪检测显示,与对照组相比,感染后48小时Eca-109细胞G2期细胞明显减少（P〈0.01）,S期细胞明显增多（P〈0.01）;转染后的Eca-109细胞中凋亡细胞明显增多,感染48h后达16.0%。结论：过表达NDRG2可明显抑制人食管鳞癌细胞Eca-109的增殖并诱导细胞凋亡。

Influence of NDRG2 on proliferation and apoptosis of human esophageal cell line Eca-109

Objective：To investigate the function of candidate tumor suppressor gene NDRG2 on proliferation and apoptosis of human esophageal cancer cell line Eca-109.Methods：Eca-109 cells were transfected with adenovirus vector containing NDRG2 gene. The expression of message RNA and protein of NDRG2 were detected by RT-PCR and Western blot respectively. The cell growth curve was drawn by methyl thiazolyl tetrazolium assay（MTT）. Flow cytometry （FCM） was adopted to analyze quantitatively the cell cycle and apoptosis.Results：NDRG2 expression was significantly increased in both mRNA and protein levels in Eca-109 after transfecting with adenovirus vector carrying NDRG2 gene. As compared with normal Eca-109 cells, the growth was markedly slowed down in NDRG2 over expressing cells. The G2 phase cells were decreased significantly （P〈0.01）, whereas S phase cells were obviously increased. Moreover, apoptotic cells which presenting typical morphologic changes of cell apoptosis, reached 16% after adenovirus transfection.Conclusion：NDRG2 gene can significantly inhibit cell growth and markedly induce the apoptosis of Eca-109 cells in vitro.