| 人外周血T细胞主要功能亚群的分离及某些性质的研究 |
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| 引用本文： | 白炎,黎燕,张双喜,陈勇.人外周血T细胞主要功能亚群的分离及某些性质的研究[J].中国免疫学杂志,1988(6). |
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| 作者姓名： | 白炎 黎燕 张双喜 陈勇 |
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| 作者单位： | 军事医学科学院基础医学研究所 北京
(白炎，黎燕，张双喜)，军事医学科学院基础医学研究所 北京(陈勇) |
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| 摘 要： | 用本实验室自制CD_4类抗体T_(4a)T_(4b)和CD_3类抗体T_(3b)及流式细胞荧光激活分类仪(FACS440)将正常人外周血分离成T_(4a)(T_(4b))~+、T_(4a)(T_(4b))~-、T_(8b)~+、T_(8b)~-细胞。对所分离的细胞纯度分析表明.其纯度达95%以上.用CD_4、CD_(?)类对所分离细胞进行双染分析,发现T_(4a)(T_(4b)~+、T_(4a)(T_(4b))~-,T_(8b)~+中不含T_(4a)(T_(ab))~-、T_(8b)细胞.同样在阴性细胞中,阳性细胞的含量不超过2.2%.用此高度纯化的T_(4a)(T_(4b))~+、T_(8b)细胞,观察PHA诱导分泌IL-2的情况.发现仅T_(4a)(T_(4b)_~+细胞分泌IL-2.在本实验条件下,T_(8b)细胞不分泌IL-2.
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| 关 键 词： | CD_4类抗体 CD_2类抗体 流式细胞荧光激活分类仪 |
SEPARATION AND IL-2 SECRETION OF TWO MAJOR T CELL SUBSETS |
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| Bai Yan,et al Institute of Basic Medical Sciences,Military Academy of Medical Sciences,Beijing.SEPARATION AND IL-2 SECRETION OF TWO MAJOR T CELL SUBSETS[J].Chinese Journal of Immunology,1988(6). |
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| Authors: | Bai Yan Institute of Basic Medical Sciences Military Academy of Medical Sciences Beijing |
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| Abstract: | T4a(T4b)~+,T4a(T4b)~-,T8b~+,T8b~-cells from human peripheral bloodmonoclonal cells were separatedby CD4 McAbs(T4a,T4b),CD 8McAb(T8b) and FACS 440(BD).The purities of Th cells(T4a,T4b~+cells) as well as suppressor/killercells(T8b~+)was above 95% accordingto analysis on the separated cells.It was found that the T helpercells were the only cells whichsecreted IL-2 under our workingcondition. |
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| Keywords: | T Cell Subset IL-2 Secretion |
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