巯基烷基化壳聚糖介导piRES-hVEGF121cDNA／hBMP-4真核双表达质粒修复兔桡骨缺损的初步研究

IB的研究以巯基烷基化壳聚糖（TACS）介导的双核共表达质粒（pIRES—hVEGF121cDNA／hBMP-4）修复骨缺损的作用。方法以新西兰兔15只（30侧）为实验动物，制作桡骨中段15mm长的骨缺损实验模型，分实验组（左侧桡骨）和对照组（右侧桡骨），实验组植入明胶海绵＋双基因混悬液，对照组植入明胶海绵＋等量生理盐水，并于术后6周、8周、12周对骨缺损区进行大体观察、X线观察、X线阻射密度测定及组织形态学观察，图像分析骨小梁的生成数量。结果实验组在12周内骨缺损完全修复，且同时期内新生骨的数量和质量显著优于对照组：对照组骨缺损主要由纤维结缔组织填充。结论TACS介导的pIRES—hVEGF121cDNA／hBMP-4真核双表达基因具有良好的促进骨缺损修复的能力。

A Preliminary Study of Thiolated N-alkylated Chitosan-mediated pIRES-hVEGF121cDNA/hBMP-4 Eukaryotic Coexpression Plasmid to Repair Radial Bone Defects in Rabbits

Objective Evaluate the effect of Thiolated N-alkylated chitosan-mediated plRES-hVEGF121cDNA/hBMP-4 eukaryotic co-expression plasmid in repairing radial bone defects of rabbits. Methods Thirty radial bone defects （15 mm length） from 15 New Zealand rabbits were divided into 2 groups： the experimental group （left radial bone） and the control group （right radial bone）. In the experimental group, defect was repaired by gelatin sponge ＋ eukaryon co-expression plasmid and in the control group by gelatin sponge ＋ Sodium Chloride. The osteogenesis in the bone defect was evaluated by radiograph and histology at 6, 8, and 12 weeks after the surgery. The new bone formation was measured by radio-density quantification and image analysis. Results The bone defect was completely repaired in the experimental group 12 weeks after the implantation. The results were better than the control groups. The bone defects in the control group was filled only with fibrous and connective tissues at 12 weeks after the surgery. Conclusion Thiolated N-alkylated chitosan mediated plRES-hVEGF121cDNA/hBMP-4 eukaryotic co-expression plasmid can effectively repair radial bone defects.