曲尼司特微球对兔晶状体后囊膜混浊的抑制作用

目的观察曲尼司特微球(tranilastMS)对兔眼晶状体超声乳化术后后囊膜混浊的抑制作用。方法将47只兔眼随机分为8组,均行超声乳化晶状体摘除术,术毕除空白对照组外均一次性囊袋内注入含不同药物的0.1ml混悬介质,所含物分别为2mg、1mg、0.5mgtranilastMS,1.85mg空白微球,0.06mg地塞米松缓释剂,0.15mgtranilast原料药,0.1ml混悬介质囊袋内注入为对照组之一。术后按时检查前房反应并测量眼压。术后第1个、第2个、第3个月对术眼进行拍照,获得后囊膜混浊图像,分析后囊膜混浊指数,并进行统计学比较。术后第3个月摘取术眼行组织病理学检查。结果术后除空白对照组外,余组前房内反应均较重,持续时间较长,各组眼压无明显增高。对后囊膜混浊指数进行统计学比较显示,含tranilastMS的后囊膜混浊较轻,与其他组比较差异有显著性(P<0.05),不同浓度tranilastMS组之间差异也有显著性。光镜显示术后第3个月用药组后囊膜光滑,无明显增殖,电镜显示用药组及非用药组角膜和虹膜超微结构无明显变化。结论曲尼司特微球在实验期内可以抑制后囊膜混浊的发生和发展,作用呈浓度依赖性,对眼内其他组织无明显毒副作用。

Effect of tranilast microspheres (tranilast-MS) in preventing PCO in the rabbit

Objective To evaluate the effects of tranilast-MS on the prevention of PCO after in vivo rabbit eyes were treated with tranilast-MS.Methods Forty-seven rabbit eyes were randomly divided into 8 groups according to treatment protocols: three tranilast-MS groups (2 mg, 1 mg, 0.5 mg), microspheres without tranilast group, dexamethasone group, tranilast crystals group, medium group, and normal control group. A standard phacoemulsification operation was performed on the eyes in all of the groups, and this was followed by treatment with the solutions listed above. On 30, 60, and 90 days postoperatively, the animals were monitored in vivo for anterior reaction, intraocular pressure (IOP), and for the presence of PCO by using a high-resolution imaging analysis system. Histological examination was performed 12 weeks after surgery.Results In this study we found that, apart from the control group, the anterior chamber reaction was severe and persisted for a long time after treatment, but IOP did not increase significantly. By using a high-resolution imaging system, we found that the degree of PCO was less severe in the tranilast-MS groups than in the other groups. Moreover, there were significant differences among the tranilast-MS groups. Histological examination did not show significant LEC proliferation in the tranilast-MS groups or obvious damage to the cornea or iris in any of the groups 3 months after surgery.Conclusion Our study suggests that tranilast-MS is a potential agent for prevention of PCO and is less toxic to surrounding ocular tissue.