黄芪对梗阻性黄疸大鼠肾功能的保护作用

目的:探讨黄芪对梗阻性黄疸大鼠肾功能的保护作用及机理.方法:SD大鼠胆总管结扎后分2组,每组20只,术后分别用3 mL黄芪或生理盐水腹腔注射.假手术组20只.术后10 d、20 d(每小组n=10)心脏取血测定血浆内皮素(ET-1)、血清超氧化物歧化酶(SOD)和丙二醛(MDA)含量,同时测定血清肌酐(Cr)尿素氮(BUN)和直接胆红素(DB),对肾脏行病理形态学观察.结果:血浆ET-1、血清MDA浓度在梗阻10 d即升高且随胆道梗阻时间延长进一步升高,SOD在梗阻10 d即下降,同时伴有血清Cr、BUN、DB的升高和肾脏病理形态学的进行性改变.黄芪治疗组ET-1及MDA显著低于对照组,而SOD显著高于对照组,并能改善肾组织病理形态.结论:大鼠梗阻性黄疸时血浆ET-1升高及氧自由基损害是肾损伤的原因,黄芪通过降低ET-1水平、抗氧化作用对梗阻性黄疸大鼠肾脏起保护作用.

Protective Effect of Astragalus Injection on Renal Function in Rats with Obstructive Jaundice

Objective To evaluate the protective effects on renal function and mechanism of Astragalus injection in rats with obstructive jaundice(OJ) Methods Forty rats with ligated bile duct were at random divided into normal saline group(NS) and Astragalus injection group. The rats were administered 3 ml saline and 3 ml Astragalus injection since the second day after bile duct ligation. Twenty rats had sham-operation. The level of endothelin(ET), superoxide dismutase(SOD) and malondialdehyde(MDA) were measured in rats(n=10) on l0th and 20th days after operation. Meanwhile, the levels of serum bililubin(DB) and BUN and Cr were also determined in all the rats. Renal histological changes were observed with light microscopy. Results The levels of ET-1 and MDA as well as those of serum DB, BUN and Cr in all OJ subgroups were continually elevated. The level of SOD in OJ rats was lower than that in sham-operation group. The level of ET-1 and MDA in Astragalus injection group were lower than those in NS group, and the SOD content was higher than that in control. Astragalus injection ameliorates the pathological changes of the renal tissue. Conclusion In rats with obstructive jaundice, Astragalus injection may have the function of ameliorating renal dysfunction by reducing ET-1 and antioxidation.