胶体金标记人外周血白细胞表面 Con A 受体及其定位和定量分析

本实验用刀豆球蛋白 A-辣根过氧化物酶胶体金(Con A-HRP-G)法定量分析了戊二醛固定后的人外周血白细胞表面受体的分布和密度,可见所有白细胞以及血小板均显示具有 Con A受体。细胞表面平均每μm 周长金标 Con A 复合物的定量分析表明,细胞表面 Con A 受体标记密度因细胞类型而不同。各类血细胞 Con A 受体的标记密度依次为:分叶核中性粒细胞<单核细胞>NK 细胞>杆状核中性粒细胞>嗜碱粒细胞>嗜酸粒细胞>淋巴细胞。小淋巴细胞的密度明显多于大淋巴细胞。NK 细胞表面的受体有极性分布。以上结果说明:1.Con A 受体与细胞功能活性有关,细胞越活跃,则其表面 Con A 受体越多;2.Con A 受体可能与细胞的识别功能有关,如NK 细胞 Con A 受体呈极性分布的现象;3.Con A 受体数目与细胞的成熟程度有关,细胞越成熟,其表面 Con A 受体越多。

DISTRIBUTION AND QUANTITATIVE STUDY OF CON A RECEPTOR ON THE SURFACE OF HUMAN PERIPHERAL BLOOD LEUCOCYTES

Con A-colloidal-gold-labelled horseradish peroxidase was used to analyse thedensity and distribution of concanavalin-A (Con A) receptor sites on the surface ofisolated human peripheral blood leucocytes.The quantitative analysis of the numberof gold Con A complex per micron of leucocyte surface indicated that the extentof surface labeling was dependent on cell types.The extent of surface labeling frommore to less was:segmented neutrophil>monocyte>NK cell>band neutrophil>basophil>eosinophil>lymphocyte,and especially the small lymphocytes were moreheavily labeled than the larger ones,and the Con A receptor of NK cell exhibitedpolarization along the cell surface.These results suggest that:1.the extent of ConA receptors was related to the functional activities of the ceils,the more active thecell is,the more the Con A receptors will be;2.the receptors of Con A wereprobably related to the cell recognition,for instance,on the NK cell Con A rece-ptors showed polar distribution;3.the number of receptors of Con A was dependenton the cell differentiation,the more mature the cell is,the more the Con Areceptors are.