| 针刺神门穴及命门穴对大鼠中缝背核5-羟色胺和5-羟吲哚乙酸含量的影响 |
| |
| 引用本文： | 王慧,王旭,唐卉凌,白丽敏.针刺神门穴及命门穴对大鼠中缝背核5-羟色胺和5-羟吲哚乙酸含量的影响[J].中医杂志,2011,52(12):1038-1040. |
| |
| 作者姓名： | 王慧 王旭 唐卉凌 白丽敏 |
| |
| 作者单位： | 1. 贵阳中医学院基础部,贵州省贵阳市市东路50号,550002
2. 北京中医药大学基础医学院科研实验中心 |
| |
| 摘 要： | 目的研究针刺神门和命门穴后大鼠中缝背核5-羟色胺(5-HT)和5-羟吲哚乙酸(5-HIAA)含量变化,探讨针刺治疗失眠症的作用机制。方法将SD雄性大鼠18只随机分为对照组、针刺神门组、针刺命门组各6只,采用立体定位法将微透析探针置入大鼠中缝背核,运用微透析法收集大鼠针刺前40min,针刺后20、40、60、80、100、120min中缝背核细胞外液,用高效液相-电化学法(HPLC-ED)检测其5-HT和5-HIAA含量的变化。结果与针刺前相比,针刺大鼠神门和命门穴20min后中缝背核5-HT的水平显著下降(P<0.01),在其后2h维持在低水平;与对照组相比,大鼠针刺后2h各时相点中缝背核5-HT的水平也显著下降(P<0.01)。与针刺前的基础值及与对照组相比,针刺神门和命门穴均显著降低中缝背核5-HIAA含量(P<0.01)。结论针刺神门和命门穴能通过调节中缝背核组织中5-HT和5-HIAA含量从而起到调节睡眠的作用。
|
| 关 键 词： | 针刺 脑微透析 中缝背核 5-羟色胺 5-羟吲哚乙酸 |
Effect of Acupuncture at Shenmen (HT 7) and Mingmen (GV 4) on the Contents of 5-HT and 5-HIAA of the Dorsal Raphe Nucleus of Rats |
| |
| WANG Hui,WANG Xu,TANG Huiling,BAI Limin.Effect of Acupuncture at Shenmen (HT 7) and Mingmen (GV 4) on the Contents of 5-HT and 5-HIAA of the Dorsal Raphe Nucleus of Rats[J].Journal of Traditional Chinese Medicine,2011,52(12):1038-1040. |
| |
| Authors: | WANG Hui WANG Xu TANG Huiling BAI Limin |
| |
| Institution: | WANG Hui1,WANG Xu2,TANG Huiling2,BAI Limin2(1.Department of Preclinical Medicine,Guiyang College of Traditional Chinese Medicine,Guiyang 550002,2.College of Preclinical Medicine,Beijing University of Chinese Medicine) |
| |
| Abstract: | Objective To study the changes in the contents of 5hydroxytryptamine(5-HT) and 5-hydroxyindoleacetic acid(5-HIAA) of the dorsal raphe nucleus(DRN) of rats after acupuncture at Shenmen(HT 7) and Mingmen(GV 4) to discuss the mechanism of acupuncture treatment for insomnia.Methods Eighteen SD male rats were randomized into control group,acupuncture Shenmen(HT 7) group and acupuncture Mingmen(GV 4) group,with 6 in each.A microdialyzer probe was placed into the DRN with stereotaxic technique.The extracellular fl... |
| |
| Keywords: | acupuncture cerebral microdialysis dorsal raphe nucleus(DRN) 5-hydroxytryptamine(5-HT) 5-hydroxyindoleacetic acid(5-HIAA) |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
