| 在乳腺癌干细胞中Hedgehog信号通路相关基因表达增强 |
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| 引用本文： | 陶雅军,毛俊,张晴晴,李连宏.在乳腺癌干细胞中Hedgehog信号通路相关基因表达增强[J].基础医学与临床,2011,31(4). |
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| 作者姓名： | 陶雅军 毛俊 张晴晴 李连宏 |
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| 作者单位： | 大连医科大学病理学教研室,辽宁,大连,116044 |
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| 基金项目： | 国家自然科学基金，教育部高等学校博士学科点专项科研基金 |
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| 摘 要： | 目的 了解Hedgehog信号通路在乳腺癌发生发展中的作用.方法 用免疫磁珠法从无血清培养的乳腺癌悬浮细胞中分选CD44+CD24-细胞和非CD44+CD24-细胞,用real-time RT-PCR法检测Hedgehog信号通路主要分子$HH、PTCH1、SMO和GLI1 mRNA在细胞中的表达,用免疫组织化学法检测上述因子在乳腺癌组织中的表达.结果 分选出的CD44+CIDA-细胞约占乳腺癌悬浮细胞总数的8.25%,分选出的CD44+CD24-细胞表达干细胞标志蛋白ALDHA1和Oct-4;SHH、PTCH1、SMO和GLI1 mRNA在CD44+CD24-细胞中的表达均高于其在非CD44+CD24-细胞中的表达(P＜0.05);SMO和GLI1蛋白在三阴性乳腺癌的表达均高于非三阴性乳腺癌组织(P＜0.05).结论 在乳腺癌干细胞CD44+CD24-细胞中Hedgehog信号通路被激活,抑制癌症干细胞中Hedgehog通路的活化可能会降低或阻止乳腺癌的复发及化疗耐受.
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| 关 键 词： | Hedgehog信号通路 乳腺癌 CD44+CD24-细胞 |
Expression reinforcement of Hedgehog signaling genes in breast cancer stem cells |
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| TAO Ya-jun,MAO Jun,ZHANG Qing-qing,LI Lian-hong.Expression reinforcement of Hedgehog signaling genes in breast cancer stem cells[J].Basic Medical Sciences and Clinics,2011,31(4). |
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| Authors: | TAO Ya-jun MAO Jun ZHANG Qing-qing LI Lian-hong |
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| Institution: | TAO Ya-jun,MAO Jun,ZHANG Qing-qing,LI Lian-hong*(Dept.of Pathology,Dalian Medical University,Dalian 116044,China) |
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| Abstract: | Objective To explore the effect of Hedgehog signaling pathway in breast carcinogenesis.MethodsCD44+CD24-cells and non-CD44+CD24-cells were selected from cell suspension in the culture system of a serum-free medium by magnetic activated cell sorting system(MACS),RT-PCR was employed to detect the expression of SHH,PTCH1,SMO and GLI1 mRNA in CD44+CD24-cells and non-CD44+CD24-cells,immunohistochemistry test was applied to study the expressions of SHH,PTCH1,SMO and GLI1 protein in breast cancer.ResultsCD44+CD24-... |
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| Keywords: | Hedgehog signaling breast cancer CD44 CD24-cell |
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