| microRNA-21可调控Flk1+间充质干细胞的迁移 |
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| 引用本文： | 王世华,黄姗,边春景,赵春华.microRNA-21可调控Flk1+间充质干细胞的迁移[J].基础医学与临床,2011,31(4). |
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| 作者姓名： | 王世华 黄姗 边春景 赵春华 |
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| 作者单位： | 中国医学科学院北京协和医学院基础医学研究所组织工程研究中心,北京,100005 |
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| 基金项目： | 国家高技术研究发展计划(863)项目 |
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| 摘 要： | 目的 用microRNA-21抑制刺转染Flk1+间充质干细胞(MSC),探讨microRNA-21对Flk1+MSC(MSC)迁移的影响.方法 在脂质体介导下用microRNA-21抑制剂瞬时转染Flk1+MSC,用Transweil技术检测细胞迁移,用Real-time PCR技术检测micro-RNA的表达.结果 与对照组相比,实验组中microRNA-21表达明显下调,实验组细胞在12、16和20 h迁移率分别是对照组的77.5%、71.3%和69.8%.结论 microRNA-21可调控Flk1+MSC的迁移.
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| 关 键 词： | 间充质干细胞 迁移 |
MicroRNA-21 regulates the migration of Flk1 + mesenchymal stem cells |
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| WANG Shi-hua,HUANG Shan,BIAN Chun-jing,ZHAO Chun-hua.MicroRNA-21 regulates the migration of Flk1 + mesenchymal stem cells[J].Basic Medical Sciences and Clinics,2011,31(4). |
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| Authors: | WANG Shi-hua HUANG Shan BIAN Chun-jing ZHAO Chun-hua |
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| Institution: | WANG Shi-hua,HUANG Shan,BIAN Chun-jing,ZHAO Chun-hua*(Research Center of Tissue Engineering,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100005,China) |
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| Abstract: | Objective To evaluate the effect of microRNA-21 on migration of Flk1+ mesenchymal stem cells(MSCs).Methods Flk1 +MSCs were Transfected with microRNA-21 inhibitor using Lipofectamin2000 and their migratory activity was evaluated by Transwell.Real-time PCR was applied to detect expression of microRNA.Results Compared to control group,the expression of microRNA-21 was significantly reduced in the inhibitor-transfected group.Moreover,the number of cells migrated through Transwell in the inhibitor-transfected gr... |
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| Keywords: | microRNA-21 |
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