脑源性神经营养因子转基因细胞移植和神经节苷脂对大鼠脊髓损伤N-甲基-D-天门冬氨酸受体的影响

目的研究脑源性神经营养因子（BDNF）转基因细胞移植和神经节苷脂（GM-1）对大鼠脊髓损伤N-甲基-D-天门冬氨酸（NMDA）受体的影响。方法将成年大鼠分为四组，A组：单纯脊髓损伤组；B组：脊髓损伤＋AxCA-BDNF基因转染的成肌细胞移植组；C组：脊髓损伤＋GM-1组；D组：脊髓损伤＋AxCA-BDNF基因转染的成肌细胞移植＋GM-1组。伤后1、3、7、14d采用[^3H]MK-801放射性配基分析法检测大鼠损伤后脊髓NMDA受体的变化。结果发现各组[^3H]MK-801放射性配基分析最大结合容量都有不同程度的减少，其减少程度顺序是A组〉B组〉C组〉D组。结论应用GM-1和BDNF转基因细胞移植后可以通过影响脊髓NMDA受体，从而减轻脊髓继发性损伤。

Effect of intraspinal grafting of adenovirus-mediated BDNF ex vivo transgene myoblasts and GM-1 gangliosides on N-methyl-D-aspartate (NMDA) receptors after cervical spinal cord contusion in rats

Objective To investigate the effect of intraspinal grafting of adenovirus- mediated BDNF ex vivo transgene myoblasts and GM- 1 gangliosides on N- methyl- D- aspartate (NMDA) receptors after spinal cord injury (SCI) in rats. Methods The experimental rats were divided into 4 groups: Group A (spinal cord contusion), Group B (contusion plus transplants of ex vivo transgene myoblasts to injured site), Group C (contusion plus GM- 1) and Group D (contusion plus transplants of ex vivo transgene myoblasts plus GM- 1). 1, 3, 7 and 14 days after contusion, antibodies to the radioligand assay of [3H]MK- 801 were used to assess NMDA receptors in the spinal cord. Results The radioligand assay of [3H]MK- 801 was found to reduce in the injured cord segments in all the groups. As for the reduction, Group A >Group B >Group C >Group D (P< 0.05). Conclusion The GM- 1 and BDNF transgene myoblasts must be affected by a mechanism that can withstand enhanced function of NMDA receptor and thus attenuate secondary SCI