脾酪氨酸激酶对大鼠肺血管平滑肌细胞增殖的作用

目的：研究脾酪氨酸激酶（spleen tyrosine kinase, Syk）在血小板源性生长因子BB（platelet-derived growth factor-BB, PDGF-BB）诱导大鼠肺血管平滑肌细胞（PVSMCs）增殖中的作用。方法：体外培养雄性Sprague-Dawley大鼠PVSMCs，经鉴定后选用3~5代细胞，经PDGF-BB诱导细胞增殖后，用3种不同剂量Syk选择性抑制剂piceatannol干预，以MTT比色法测定细胞增殖，3H-TdR掺入法检测细胞DNA合成，流式细胞仪检测细胞周期，荧光定量PCR、Western blot分别检测细胞Syk mRNA和蛋白表达水平。结果：PDGF-BB刺激后，Syk蛋白表达水平明显增高，细胞显著增殖；经Syk抑制剂piceatannol干预后，随着Syk mRNA和蛋白表达水平的降低，细胞的增殖受到明显抑制，其抑制作用与剂量大小具有相关性。结论：在PDGF-BB诱导下的大鼠肺血管平滑肌细胞增殖中，Syk可能起促进作用。 ［中国当代儿科杂志，2010，12（11）：886-890］

Effect of spleen tyrosine kinase on the proliferation of pulmonary vascular smooth muscle cells in rats

Objective To investigate the role of spleen tyrosine kinase(Syk) in rat pulmonary vascular smooth muscle cells(PVSMCs) proliferation induced by platelet-derived growth factor-BB(PDGF-BB).Methods PVSMCs from male Sprague-Dawley rats were cultured in vitro and the cells of passages 3-5 were used in the experiment.PVSMCs were stimulated by PDGF-BB and were treated with three different doses of piceatannol,a Syk selective inhibitor.Cell proliferation was assessed by methyl thiazolyl tetrazolium(MTT) assay.DNA synthesis was measured by 3H-thymidine incorporation(3H-TdR).Cellular cycle was observed by flow cytometry.Syk mRNA and protein expression were detected using real-time quantitative PCR and Western blot,respectively.Results The expression of Syk protein of PVSMCs was significantly up-regulated following PDGF-BB stimulation.PDGF-BB stimulation dramatically increased PVSMCs proliferation.After piceatannol treatment,both Syk mRNA and protein expression decreased and the proliferation of PVSMCs was inhibited in a dose-dependent manner.Conclusions Syk may promote PVSMCs proliferation induced by PDGF-BB.