补肾法诱导间充质干细胞向神经方向分化研究

目的　在干细胞具先天之精属性新理论指导下 ,探讨补肾中药龟板对间充质干细胞的体外诱导作用。方法　体外分离培养间充质干细胞 ,以龟板水煎液对其进行体外诱导 ,免疫组化鉴定NSE、Nestin、GFAP等标志。结果　龟板可体外诱导间充质干细胞转分化为神经干细胞 ,但还没有被诱导为神经元样细胞与星形胶质细胞。结论　补肾法可促进干细胞之间的相互转换 ,初步验证了干细胞具先天之精属性新理论。

INDUCTION OF THE DIFFERENTIATION OF MESENCHYMAL STEM CELLS INTO CELLS OF NERVE SYSTEM BY INVIGORATING THE KIDNEYS

Objective To investigate the differentiation of mesenchymal stem cells(MSC) into nerve system cells according to our new viewpoint that stem cells have the same properties of congenital essence. Methods MSCs were separated and cultured in vitro and were induced by the decoction of tortoise plastron (TP), a Chinese traditional medicine with roles of invigorating the kidneys. NSE, NESTIN, GFAP were detected by immunohistochemistry. Results TP can induce MSCs to differentiate into neural stem cells, not neural like cells and astrocytes. Conclusion This result shows that invigorating the kidneys can promote the interconversion between the stem cells, and it preliminary validate our new viewpoint that stem cells have the same properties of congenital essence.