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| 质粒DNA对大鼠骨骼肌肌质网Ca2+转运的影响 | |
| 引用本文： | 赵文,李载权,姜志胜,汤健,贾弘禔,刘乃奎,唐朝枢.质粒DNA对大鼠骨骼肌肌质网Ca2+转运的影响[J].北京大学学报(医学版),1999,31(4). |
| 作者姓名： | 赵文 李载权 姜志胜 汤健 贾弘禔 刘乃奎 唐朝枢 |
| 作者单位： | 1. 北京医科大学心血管基础研究所 2. 第一医院心血管研究所 3. 生物化学与分子生物学系,北京,100083 |
| 摘 要： | 目的：观察ＤＮＡ与骨骼肌肌质网蛋白结合后对肌质网功能的影响。方法：应用差速离心分离大鼠骨骼肌肌质网，并用４５ＣａＣｌ２作为示踪剂，观察质粒ＤＮＡｐｃＤＮＡ３和ｐｃＤＮＡ３／ＡＮＦ对肌质网钙转运功能的影响。结果：两种质粒均可明显增加肌质网Ｃａ２＋摄取，呈明显的量效关系，并且也可促进肌质网钙释放。结论：外源质粒ＤＮＡ可影响肌质网钙转运功能。 |
| 关 键 词： | 质粒 DNA结合蛋白质类 钙/代谢 肌肉骨骼系统 |
Effects of plasmid DNA on sarcoplasmic reticulum Ca2+ transport in rat skeletal muscle |
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| ZHAO Wen,LI Zai-Quan,JIANG Zhi-Sheng,TANG Jian,JIA Hong-Ti,LIU Nai-Kui,TANG Chao-Shu.Effects of plasmid DNA on sarcoplasmic reticulum Ca2+ transport in rat skeletal muscle[J].Journal of Peking University:Health Sciences,1999,31(4). | |
| Authors: | ZHAO Wen LI Zai-Quan JIANG Zhi-Sheng TANG Jian JIA Hong-Ti LIU Nai-Kui TANG Chao-Shu |
| Abstract: | Objective: To observe the effect of plasmid DNA binding to sarcoplasmic reticulum (SR) non nuclear DNA binding proteins on SR function. Methods: SR vesicles of rat skeletal muscle were prepared by differential centrifuge. Effects of plasmids pcDNA3 and pcDNA3/ANF on SR Ca 2+ transport were investigated by using 45 CaCl 2 as tracer. Results: Both plasmids could increase SR Ca 2+ uptake significantly, which had significant dose dependent relation and increased SR Ca 2+ release. Conclusion: Plasmid DNA could influence SR Ca 2+ transport. |
| Keywords: | Plasmids DNA binding proteins Calcium/metab Musculoskeletal system |
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