K562 human leukemia cell passages differ in embryonic globin gene expression |
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Authors: | Peter T. Rowley Betsy M. Ohlsson-Wilhelm Lawrence Wisniewski Carmen B. Lozzio Bismarck B. Lozzio |
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Affiliation: | 1. Department of Medicine, University of Rochester School of Medicine, Rochester, New York, U.S.A.;2. Department of Microbiology, University of Rochester School of Medicine, Rochester, New York, U.S.A.;3. Division of Genetics, University of Rochester School of Medicine, Rochester, New York, U.S.A.;4. The Department of Medical Biology, University of Tennessee, Knoxville, Tennessee, U.S.A. |
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Abstract: | K562 is a human leukemia cell line inductible by a variety of agents for the synthesis of embryonic and fetal hemoglobins. We compared early and late passages to determine whether a change has occurred in globin synthetic pattern. Clone LA4, derived from passage 199 which had been frozen by Lozzio in 1973, was compared with clone RA6, derived from a line received from Rutherford in 1979. Globin synthetic pattern was determined by incubation with [3]leucine, separation of globins by Triton-X100 polyacrylamide gel electrophoresis, and analysis by fluorography. For RA6, hemin-induced synthesis was greatest for zeta globin but minimal for epsilon globin, whereas for LA4 it was greatest for epsilon globin but minimal for zeta globin. Both lines are pseudotriploid with three No. 11 and three No. 16 chromosomes. However only RA6 has a translocation involving the short arm of chromosome 11 which contains the locus of the beta globin gene cluster. However, translocation-associated deletion does not simply explain the deficient inducibility of epsilon synthesis because Gγ and Aγ globins, whose genes are linked to the epsilon gene, are similarly inducible in the two lines. |
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Keywords: | K562 cells leukemia hemoglobin gene expression differentiation erythropoiesis a human multipotent leukemia cell line inducible for the synthesis of hemoglobins a clone of K562 cells derived from passage 199 a clone of K562 cells derived from a passage received from Rutherford in 1979 a medium for cell culture curies per millimole absorbency at 416 nm at 1 mg solute per ml Giemsa quinicrine centromeric 4′-6-diamidino-2-phenylindole micromolar standard error short arm chromosome 6 long arm of chromosome 19 short arm of chromosome 11 Philadelphia chromosome, a chromosome 22 with a terminal deletion of the long arm found in the majority of chronic myeloid leukemic cells |
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