HBeAg阳性患者血清中Pre-S1抗原与C基因启动子变异的检测

目的 研究HBV C基因启动子(Basic Core Promoter，BCP)与前S1(Pre-S1)抗原基因变异的关系，以及BCP基因变异与HBV DNA含量的关系。方法 分别对94例HBeAg阳性乙肝患者进行酶联免疫吸附法(ELISA)检测Pre—S1抗原，PCR荧光定量技术检测HBV DNA，PCR—微板核酸杂交ELISA技术检测BCP中nt1762A—T和nt 1764G—A的基因突变。结果 在94例HBeAg阳性乙肝患者血清检测中，Pre—S1抗原阳性有65例，其中BCP阳性51例，BCP变异率为78．5％；Pre—S1抗原阴性有29例，其中BCP阳性23例，BCP变异率为79．3％。74例BCP基因突变血清中，55例(74．3％)的HBV DNA拷贝数超过了10^5 CPS／ml；而在20例非BCP基因突变血清中，只有6例(30％)的HBV DNA拷贝数超过了10^5CPS／ml。结论 e抗原阳性乙肝患者中，Pre—S1抗原的存在与BCP的变异并无相关性，但BCP基因变异与HBV DNA拷贝数则成正相关，它们都可反映乙肝病情的严重程度及其愈后、转归。

Study on pre-S1 antigen and BCP mutation in HBeAg positive HBV infected patients

Objective To investigate the relationships between BCP mutation and Pre S1 antigen in HBV patients,and to explore the pertinence between mutation and HBV DNA concentration.Methods ELISA was applied to detect pre S1 antigen,fluorescence quantitive PCR was used to quantitate the HBV DNA concentration,PCR microplate nucleotide acid hybridization ELISA was used to detect the gene mutation of 1762(A T) and 1764(G A) in BCP in 94 HBeAg positive HBV infected patients respectively.Results Sixty five pre S1 antigen positive in 94 tested samples,and BCP gene mutation had taken place in 51 of them,the positive rate was 78.5%.While only 23 appeared to be BCP positive in 29 Pre S1 antigen negative group.The mutation rate was 79.3%.And in gene mutation group,55 out of 74 specimens HBV DNA concentration were above 10 5 CPS/ml (74.3%).Meanwhile,only 6 out of 20 exceed 10 5 CPS/ml in the BCP negative group (30%).Conclusion In the HBV infected patients with HBeAg positive,the existence of Pre S1 antigen was of no relation with BCP variation.But BCP variation was directly interrelated to the HBV DNA concentration;they can both reflect the degree of disease and its consequence ending.