| 炎性痛大鼠脊髓和背根节P2X_3受体的表达变化 |
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| 引用本文： | 杜璐,张金海,阮怀珍. 炎性痛大鼠脊髓和背根节P2X_3受体的表达变化[J]. 第三军医大学学报, 2010, 32(2) |
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| 作者姓名： | 杜璐 张金海 阮怀珍 |
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| 作者单位： | 第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所,重庆,400038 |
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| 基金项目： | 国家自然科学基金(30672022)~~ |
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| 摘 要： | 目的观察足底皮下注射角叉菜胶致炎后大鼠行为学及脊髓背角和背根神经节(dorsal root ganglion,DRG)P2X3受体表达的变化。方法通过热辐射刺激法及von Frey纤维丝测定大鼠痛阈,应用免疫组化技术观察足底皮下注射角叉菜胶后不同时间其相应节段(L4～L6)脊髓及背根神经节P2X3受体表达的变化。结果足底皮下注射角叉菜胶后动物出现热痛敏和机械性痛敏,热痛阈和机械痛阈均下降,于12 h达最低,72 h后恢复至正常水平。免疫组化显示相应节段(L4～L6)脊髓背角P2X3受体免疫反应在注射角叉菜胶24 h后明显增强(P<0.01),72 h表达强度最高,168 h恢复至正常,并且相应节段背根神经节P2X3受体阳性神经元数12 h开始明显增加(P<0.01),持续至72 h,168 h恢复至正常。结论角叉菜胶所致炎性痛大鼠,脊髓背角和背根神经节P2X3受体活化,表达明显上调,表明P2X3受体在炎性痛的发生、发展中起重要作用。
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| 关 键 词： | P2X3受体 背根神经节 脊髓 角叉菜胶 疼痛 |
Expression change of P2X_3 receptor in spinal dorsal horn and dorsal root ganglion in inflammatory pain rats |
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| Du Lu,Zhang Jinhai,Ruan Huaizhen. Expression change of P2X_3 receptor in spinal dorsal horn and dorsal root ganglion in inflammatory pain rats[J]. Acta Academiae Medicinae Militaris Tertiae, 2010, 32(2) |
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| Authors: | Du Lu Zhang Jinhai Ruan Huaizhen |
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| Affiliation: | Department of Neurobiology;College of Basic Medical Sciences;Third Military Medical University;Chongqing;400038;China |
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| Abstract: | Objective To investigate the expression change of P2X3 receptor in spinal dorsal horn and dorsal root ganglion(DRG) in rats with experimental inflammatory pain.Methods Totally 30 SD rats received a subcutaneous injection of carrageenan in the right paw to establish inflammatory pain model,and then divided randomly and equally into 5 groups,that is 6,12,24,72 and 168 h after injection.Another 6 rats undergoing a subcutaneous injection of normal saline in the right paw served as control.Pain threshold was ass... |
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| Keywords: | P2X3 receptor dorsal root ganglion spinal cord carrageenan pain |
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