| Effects of paclitaxel on cell proliferation and apoptosis and its mechanism in human lung adenocarcinoma A549 cells |
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| 作者姓名： | Baoan Gao Chunling Du Wenbo Ding Shixiong Chen and Jun Yang The First College of Clinical Medical Science Three Gorges University Yichang China |
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| 作者单位： | Baoan Gao1，2，Chunling Du3，Wenbo Ding1，2，Shixiong Chen1，2 and Jun Yang1，4 1The First College of Clinical Medical Science，Three Gorges University，Yichang 443003，China; 2Department of Respiratory Medicine，Yichang Central People’s Hospital，Yichang 443003，China; 3Department of Respiratory Medicine，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology，Wuhan 430030，China; 4Department of Cardiology，Yichang Central People’s Hospital，Yichang 443003，China |
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| 摘 要： | INTRODUCTION Primary bronchogenic carcinoma is one of thecommonest malignancy tumors in the world.The incidence and mortality rate are rising in recent30 years. Adenocarcinoma accounts for about 25%of all primary bronchogenic carcinomas, the curativerate is not ideal by traditional chemotherapy and ra-diotherapy1-4]. Paclitaxel is a extensive anti-cancerdrug whose mechanism is anti-microtubules and sta-bilizes cell microtubules specially, and has also beenused widely in ovarian cancer, b…
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| 关 键 词： | 肺腺癌 化疗 A549细胞 细胞增殖 细胞凋亡 细胞周期 |
Effects of paclitaxel on cell proliferation and apoptosis and its mechanism in human lung adenocarcinoma A549 cells |
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| Baoan Gao,Chunling Du,Wenbo Ding,Shixiong Chen,Jun Yang.Effects of paclitaxel on cell proliferation and apoptosis and its mechanism in human lung adenocarcinoma A549 cells[J].Journal of Nanjing Medical University,2006,20(6):360-364. |
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| Authors: | Baoan Gao Chunling Du Wenbo Ding Shixiong Chen Jun Yang |
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| Abstract: | Objective: To investigate the effect of paclitaxel on cell proliferation and apoptosis of human lung adenocarcinoma A549 cells line and its mechanism in vitro. Methods: Cell growth inhibition of paclitaxel on A549 cells was analyzed by MTT assay. Cell apoptosis was detected by DNA cytofluorometry, Hoechst33258 staining when treated with paclitaxel for 48hours. Meanwhile, Cell cycle and apoptotic rate were analyzed by flow cytometry. The protein expressions of Bax and Bcl-2 were studied by Western Blot. Results: Paclitaxel inhibited the proliferation of A549 cells in a time-and dose-dependant manner.Hoechst33258 staining indicated that apoptosis was induced by paclitaxel. After treated for 48 hours, cell apoptosis rates of 25nmol/L, 50 nmol/L and 100 nmol/L paclitaxel groups were 11.52 ± 1.94% ,17.73 ± 2.53%, and 29.32 ± 5.51% respectively,which were significantly higher than those of control group 5.88 ± 1.07%(all P ＜ 0.01 ), and apoptosis rate increased in dose-dependant manner. Meanwhile, G2/M stage cell percentage of 25 nmol/L, 50 nmol/L and 100 nmol/L paclitaxel groups were 42.52± 6.25%, 40.46 ± 5.81%, and 35.34 ± 6.17% respectively,which were significantly higher than that of control group 22.32 ±3.30%(all P ＜ 0.01 ); Western blot showed that paclitaxel increased the expression of Bax and decreased the expression of Bcl-2 in dose-dependant manner. Conclusion: Paclitaxel can inhibit A549 cell proliferation in a time- and dose-dependant manner. Its mechanism may be related to arresting cell cycle in G2/M stage and induce cell apoptosis by up-modulating Bax expression and down-modulating Bcl-2 expression. |
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| Keywords: | paclitaxel cell cycle apoptosis human lung adenocarcinoma A549 cells |
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