凝胶样支架材料对三维培养的人牙髓干细胞增殖的影响

目的　评价牙髓组织工程中不同浓度的两种可注射性凝胶样支架材料对牙髓干细胞增殖的影响。方法：配制不同浓度的I型胶原、肽段水凝胶（Puramatrix）支架材料，将牙髓干细胞分别接种于各组支架材料上；CCK-8方法检测细胞增殖情况；活死细胞染色观察细胞数量及形态。结果　接种在各浓度支架材料上的牙髓干细胞均生长良好，其中1%的胶原支架和0.25%的水凝胶支架对牙髓干细胞的增殖有明显的促进作用；活死细胞染色观察牙髓干细胞在各浓度的两种支架材料中均生长良好，细胞数量随培养时间的延长呈递增趋势；细胞充分伸展，呈纺锤型。

Effects of Gel - like Scaffold on Proliferation of Human Dental Pulp Stem Cells.

To evaluate the effects of two kinds of injectable gel-like scaffolds with different concentrations on the proliferation of dental pulp stem cells in dental pulp tissue engineering. Methods:Different concentrations of type I collagen and peptide hydrogel scaffolds were prepared, and dental pulp stem cells were inoculated on each group of scaffolds respectively. Cell proliferation was detected by CCK-8 assay; cell number and morphology were observed by staining with dead cells. Results The dental pulp stem cells inoculated with different concentrations of scaffolds all grew well. 1% collagen scaffolds and 0.25% hydrogel scaffolds could promote the proliferation of dental pulp stem cells. The viable cells stained dental pulp stem cells Both scaffold materials at various concentrations grew well, and the number of cells showed an increasing trend with the prolongation of culture time. The cells were fully extended and showed a spindle type.