新生血管性青光眼患者房水中血小板反应蛋白－1的表达及其与血管内皮生长因子相关关系的临床研究

目的检测新生血管性青光眼(NVG)与年龄相关性白内障患者房水中血小板反应蛋白－1(TSP－1)的表达情况，并分析NVG患者房水中的TSP－1与血管内皮生长因子(VEGF)的关系。 方法纳入30例(30只眼)NVG患者(NVG患者组)以及30例(30只眼)年龄相关性白内障患者(对照组)。所有NVG患者在行抗青光眼手术前抽取房水，同时在白内障手术时抽取年龄相关性白内障患者房水。采用酶联免疫吸附试验检测房水以及血液中TSP－1与VEGF的浓度。房水和血液中VEGF浓度和TSP－1浓度采用均数±标准差( ±s)表示，采用独立样本t检验进行组间比较。各细胞因子之间、细胞因子浓度与年龄、眼压之间的相关性用采用Spearman's相关分析。 结果NVG组患者房水中TSP－1浓度为(15.47±2.28) ng/ml，而对照组患者房水中TSP－1浓度为(2.28±1.59)ng/ml，NVG组患者房水中TSP－1浓度明显高于对照组，差异有统计学意义(t＝12.70，P<0.05)。NVG组患者与对照组患者房水中VEGF浓度分别为(3355.81±2284.54)pg/ml及(262.68±125.66 )pg/ml，差异有统计学意义(t＝7.41，P<0.05)。NVG组患者血浆中TSP－1浓度为(0.65±0.27)ng/ml，对照组患者血浆中TSP－1浓度为(0.57±0.25)ng/ml，两组之间浓度相当，差异无统计学意义(t＝1.16，P>0.05)。NVG组患者的VEGF血浆浓度为(13.01±7.60)pg/ml，对照组患者的VEGF血浆浓度为(14.62±6.96)pg/ml。二者比较，差异无统计学意义(t＝－0.86，P>0.05)。NVG患者房水中TSP－1与VEGF浓度呈显著正相关(r＝0.479，P<0.05)。在NVG组中，不管是TSP－1因子还是VEGF，其房水中的浓度与血浆中的浓度均无显著相关性。同样，在对照组中，房水TSP－1浓度与血浆TSP－1浓度无相关性，且房水VEGF浓度与血浆VEGF浓度亦无相关性。 结论NVG患者房水中TSP－1与VEGF表达明显上调，明显上调的TSP－1与VEGF呈现显著正相关。

Correlation between the levels of TSP-1 and VEGF in aqueous humor of patients with neovascular glaucoma

ObjectiveTo detect new vascular glaucoma(NVG) platelet response eggs in aqueous humor of patients with age-related cataract White-1 (TSP-1) Expression of the situation, and analysis of NVG patients in the aqueous humor TSP-1 and the relationship between vascular endothelial growth factor (VEGF). Methods30 NVG patients (NVG group) and 30 age-associated cataract patients (control group) were included. All NVG patients were treated with atrial water before anti-glaucoma surgery and aged cataract surgery. Using enzyme-linked immunosorbent assay to detect aqueous humor and TSP-1 and the VEGF concentration in the blood. Fluid and blood VEGF concentration and the concentration of TSP-1 using the mean±the standard deviation( ±s) the independent sample t test was used for inter-group comparison. Spearman's correlation analysis was used to analyze the correlation between various cytokines, cytokine concentration, age and intraocular pressure. ResultsNVG group patients TSP-1 concentration in aqueous humor(15.47±2.28)ng/ml the TSP-1 concentration in aqueous humor as the control group patients(2.28±1.59)ng/ml, NVG group of patients in the aqueous humor TSP-1 concentration significantly higher than the control group, the difference was statistically significant(t=12.70, P<0.05). The VEGF concentrations in aqueous humor of NVG group and control group were, respectively (3355.81±2284.54)pg/ml and(262.68±125.66)pg/ml, The difference was statistically significant(t=7.41, P<0.05). NVG TSP-1 concentration in plasma for patients with group(0.65 ±0.27)ng/ml, the control group patients for TSP -1 in plasma concentration (0.57 ±0.25)ng/ml, concentration between the two groups, there was no statistically significant difference(t=1.16, P>0.05). Plasma concentrations of VEGF were pg/ml in the NVG group and pg/ml in the control group (14.62 ± 6.96). The two comparison, there was no statistically significant difference(t=0.86, P>0.05). NVG patients TSP-1 and VEGF concentration in aqueous humor was significantly positively related to(r=0.479, P<0.05). In the NVG group, there was no significant correlation between the concentration of rh and the plasma concentration, no matter TSP-1 factor or VEGF. Similarly, in the control group, the concentration of atrial water TSP-1 was not correlated with plasma TSP-1, and the concentration of atrial water VEGF was not correlated with plasma VEGF. ConclusionThe expression of TSP-1 and VEGF was significantly up-regulated in NVG patients, while the expression of TSP-1 was significantly positively correlated with VEGF.