湖北巴戟天对D-半乳糖所致衰老模型小鼠的抗衰老作用

目的：研究湖北巴戟天是否对D-半乳糖所致衰老模型小鼠具有抗衰老作用。方法：60只昆明种雄性小鼠随机分成6组，分别为正常组，模型组，维生素E（VitE）阳性对照组（100 mg·kg^-1·d^-1）和湖北巴戟天醇提物低、中、高3个剂量组（15，30，45 mg·kg^-1·d^-1）。除正常组颈背部皮下注射等体积生理盐水外，其余各组颈背部皮下注射D-半乳糖（150 mg·kg^-1·d^-1）建立衰老模型，为期60 d。统计各组小鼠在跳台和Morris水迷宫实验中错误次数或穿越次数，计算各组小鼠的脏器指数，测量血清及脑组织中SOD、CAT、GSH-Px活力和MDA含量，并测定各组小鼠脑细胞凋亡指数。结果：湖北巴戟天醇提物3个剂量组均能减少跳台错误次数（P<0.05或P<0.01），增加水迷宫穿越次数（P<0.05或P<0.01）；升高小鼠血清及脑组织中SOD、CAT、GSH-Px活力（P<0.01），降低MDA含量（P<0.05）；提高衰老小鼠脑、胸腺、脾脏和睾丸及附睾指数（P<0.05或P<0.01），减少细胞凋亡（P<0.01），呈剂量依赖性。结论：湖北巴戟天对D-半乳糖所致衰老模型小鼠具有较好的抗衰老作用。

Anti-aging effect of Damnacanthus of ficinarum Huang on subacute aging mice induced by D-galactose

OBJECTIVE To investigate the anti-aging effects of Damnacanthus of ficinarum Huang (DOH) on subacute aging mice induced by D-galactose.METHODS Sixty mice were randomly divided into six groups:normal group,model group,vitamin E (VitE) positive control group (100 mg·kg^-1·d^-1),three treatment groups with DOH (15,30,45 mg·kg^-1·d^-1).The aging models were established by subcutaneously injecting D-galactose (150 mg·kg^-1·d^-1) in mice except those in normal group which were treated with the equal volume of physical saline for 60 days.Then mistake times in the step-down test or times of crossing the platform in Morris water maze were measured.Visceral organ index,the activities of SOD,CAT and GSH-Px and the contents of MDA in serum and brain tissue were determined.The apoptotic index of left hippocampus was also measured.RESULTS Three doses of DOH reduced mistake times (P<0.05 or P<0.01) in the step-down test and increased times of crossing the platform (P<0.05 or P<0.01),increased the activities of SOD,CAT and GSH-Px (P<0.01),decreased the contents of MDA (P<0.05) in serum and brain tissues,and improved the visceral organ index of brain,spleen,liver,testis and epididymis (P<0.05 or P<0.01) and decreased the apoptotic index of left hippocampus (P<0.01) in a dose dependent manner compared to the model group.CONCLUSION DOH shows significant anti-aging effects on subacute aging mice induced by D-galactose.