基于脑区特异性不同剂量冰片与川芎配伍抗脑缺血的研究

目的：观察不同剂量冰片配伍川芎对不同脑区缺血的保护作用。方法：复制大鼠全脑缺血-再灌注模型，观察不同剂量冰片（0.01， 0.02， 0.04， 0.08， 0.16， 0.32， 0.64， 1.28 g·kg^-1）联合川芎对大鼠皮层、海马、下丘脑和纹状体四个脑区梗死率，脑组织IL-1β、 IL-6、TNF-α含量和组织学评分的影响。结果：川芎单用能显著改善皮层区TNF-α含量。其配伍冰片后对四个脑区的梗死率，IL-1β、 IL-6、TNF-α含量和组织学评分均表现出更好的疗效。另外针对不同脑区，两药配伍疗效比较为皮层 > 海马、纹状体 > 下丘脑。针对冰片剂量，两药配伍疗效比较为0.08 g·kg^-1 > 0.16 g·kg^-1 > 0.32 g·kg^-1。结论：川芎和冰片配伍可以对缺血脑组织产生脑区特异性改善，而且这一改善作用与冰片剂量密切相关。

Research on the synergy of different doses of borneol and Chuanxiong Rhizoma on the anti-ischemnia effect in different brain regions

OBJECTIVE To investigate the enhancement effects of borneol at different doses on the anti-ischemnia of Chuanxiong Rhizoma (CR) in different brain regions.METHODS After the model of cerebral ischemia-reperfusion was established, the effect of CR combined with different doses of borneol was evaluated based on brain infarct, levels of IL-1β, IL-6, TNF-α and nissl staining.RESULTS Monotherapy of CR only improved TNF-α content in cortex, but the combined therapy of CR and borneol produced better improvements in infarct area, levels of IL-1β, IL-6, TNF-α and nissl staining in each brain region. Moreover, the therapeutic effects according to the brain regions were cortex > hippocampus, striatum > hypothalamus, the therapeutic effects according to the borneol dose were 0.08 g·kg^-1 > 0.16 g·kg^-1 > 0.32 g·kg^-1.CONCLUSION The combining therapy of CR and borneol induces a better improvement than monotherapy of CR in a region-specific manner, which is closely related to the dose of borneol.