慢性牙周炎栖牙密螺旋体和牙周袋内硫化物水平的相关性研究

目的 研究慢性牙周炎病人牙周袋内栖牙密螺旋体和牙周袋内硫化物水平的关系。方法 临床上选取17例诊断为慢性牙周炎的病人，采用金刚探针/牙周诊断仪检测牙周袋内硫化物水平，记录牙周袋探诊深度、临床附着丧失以及探诊出血相关牙周指标。同时，采用16S rRNA PCR检测相同位点的栖牙密螺旋体。结果 慢性牙周炎病人栖牙密螺旋体检出率为88.2%，硫化物阳性位点和硫化物阴性位点中栖牙密螺旋体的检出率分别为68.5%和43.2%。硫化物阳性位点与阴性位点中牙周附着丧失（clinical attachment loss, CAL）平均值分别为（2.84±2.33）mm和（1.83±1.60）mm，两者差异有统计学意义（P<0.01）。硫化物阳性位点与阴性位点牙周探诊深度（probing depth, PD）平均值分别为（4.20±1.57）mm和（3.83±1.30）mm，两者差异无统计学意义(P＞0.05)。硫化物阳性位点中牙周探诊出血（bleeding on probing, BOP）阳性检出为率92.5%，大于硫化物阴性位点（75.8%），两者差异有统计学意义（P<0.01）。结论 慢性牙周炎病人牙周袋内的硫化物水平能反映牙周栖牙密螺旋体分布情况，与牙周附着丧失存在相关性。

The relationship between Treponema denticola and periodontal pocket sulfide levels in chronic periodontitis

Objective To study the relationship between Treponema denticola(Td) and sulfide levels (SUL) in periodontal pocket of the patients with chronic periodontitis. Methods Seventeen patients with chronic periodontitis were included, sulfide levels of periodontal pocket, probing depth, clinical attachment loss, and bleeding on probing were measured by Diamond Probe /Perio 2000 system. Meanwhile, Treponema denticola was detected by 16S rRNA PCR. Results The detection rate of Treponema denticola was 88.2% in the patients with chronic periodontitis. The detection rates of Treponema denticola in sulfide-negative and sulfide-positive sites were 68.5% and 43.2%, respectively, which was statistically significant between the two groups (P<0.01). The mean values of clinical attachment loss in sulfide-positive and sulfide-negative sites were (2.84±2.33) mm and (1.83±1.60) mm, respectively, which was statistically significant between the two groups(P<0.01). The mean values of probing depth in sulfide-positive and sulfide-negative sites were (4.20±1.57) mm and (3.83±1.30) mm, respectively, which showed no statistical significance (P＞0.05). The detection rate of BOP(+) in sulfide-positive sites was 92.5%, greater than that of 75.8% in sulfide-negative sites, which showed significant difference between the two groups (P<0.01). Conclusion Sulfide levels in periodontal pocket of the patients with chronic periodontitis may reflect the distribution of periodontal Treponema denticola.