3D打印聚乙烯醇/纳米羟基磷灰石支架与丝素蛋白/聚乙烯醇/纳米羟基磷灰石支架的性能比较

目的　采用3D打印技术制备3D打印聚乙烯醇 /纳米羟基磷灰石支架与丝素蛋白/聚乙烯醇/纳米羟基磷灰石支架，并对其进行表征。方法　采用3D打印技术制作聚乙烯醇/纳米羟基磷灰石支架以及丝素蛋白/聚乙烯醇/纳米羟基磷灰石复合支架。进行孔隙率、扫描电镜、压缩力学性能及细胞毒性检测。 结果　①扫描电镜观察：丝素蛋白/聚乙烯醇/纳米羟基磷灰石支架结构规则，网状结构清晰，交通支连续，层层之间搭接良好，支架空隙均一。相同倍数下，聚乙烯醇/纳米羟基磷灰石支架网状结构连续性较差。②压缩力学性能：相同应力情况下（10 MPa），3D打印丝素蛋白/聚乙烯醇/纳米羟基磷灰石支架的应变大于3D打印聚乙烯醇/纳米羟基磷灰石支架。③孔隙率：3D打印丝素蛋白/聚乙烯醇/纳米羟基磷灰石支架的孔隙率大于3D打印聚乙烯醇/纳米羟基磷灰石支架。④细胞毒性检测：不同时间点两组支架的细胞增殖率无明显差别。结论　结果表明：3D打印聚乙烯醇 /纳米羟基磷灰石支架与丝素蛋白/聚乙烯醇/纳米羟基磷灰石支架具有良好的理化性能和细胞相容性。

Performance comparison of three-dimensional printed silk fibroin/polyvinyl alcohol/nano-hydroxyapatite scaffold and polyvinyl alcohol/nano-hydroxyapatite scaffold

Objective　 To prepare silk fibroin(SF)/polyvinyl alcohol(PVA) / nano-hydroxyapatite (nHA) scaffold and polyvinyl alcohol(PVA) / nano-hydroxyapatite (nHA) scaffold using 3D printing technology and detect their performances. Methods　3D printing technology was used to print SF/PVA/nHA scaffold and PVA/nHA scaffold. Porosity, morphology, mechanical properties and cytotoxicity of different scaffolds were detected. Results　 ①Under scanning electron microscope, SF/PVA/nHA scaffold had regular scaffold structure and clear network structure, exhibited continuous communicating branch, well-connected overlap joint, and uniform scaffold space. Under the same multiples, the network structure continuity of PVA/nHA scaffold was poorer. ②Compressive mechanical properties: Under the same stress(10 Mpa), the strain of SF/PVA/nHA scaffold was larger than that of PVA/nHA scaffold. ③The pore size of group SF/PVA/nHA scaffold was significantly larger than PVA/nHA scaffold. ④Cell toxicity detection: The deformation of SF/PVA/nHA scaffold ,under the same stress(10 MPa),was larger than PVA/nHA scaffold. There was no significant difference in cell appreciation rate between two groups of scaffolds at different time points. Conclusions　3D printing SF/PVA/nHA scaffold and PVA/nHA scaffold hold good physicochemical performance and cytocompatibility.