核酸适配体-顺铂纳米脂质体药物的制备及对人急性淋巴细胞白血病T淋巴细胞的靶向治疗作用的研究

目的： 制备核酸适配体sgc8c修饰的顺铂纳米脂质体靶向药物（sgc8c-CDDP-NLP），并研究其对人急性淋巴细胞白血病T淋巴细胞CCRF-CEM的靶向治疗作用。方法： 激光纳米粒度分析仪表征纳米颗粒的粒径，紫外分光光度计法检测顺铂的包封率，荧光分光光度计检测异硫氰酸酯（FITC）标记的纳米颗粒的荧光光谱，并用共聚焦显微观察细胞对纳米颗粒的摄取能力以及其靶向性，NB4细胞（急性早幼粒细胞白血病细胞）作为靶向阴性对照细胞；CCK-8法检测纳米颗粒对CCRF-CEM和NB4细胞活性的影响。结果： CDDP-NLP粒径分布均匀，修饰sgc8c后，粒径依然分布均匀，但是平均粒径增加为113 nm。紫外分光光度计法所测得顺铂的包封率为70.9%。sgc8c-CDDP-NLP相对CDDP-NLP明显可以高效的靶向到CCRF-CEM细胞，而很少靶向到NB4细胞上。sgc8c-CDDP-NLP对CCRF-CEM细胞发挥高效的活性抑制作用。结论： 本课题中纳米脂质体药物的制备方法简单可行，样品稳定性好，可以高效的将药物靶向到CCRF-CEM细胞表面并发挥抗癌效果。

Cis-platinum encapsulated nano liposome for aptamer directed therapy of acute lymphocytic leukemia T lymphocytes

OBJECTIVE To prepare cis-platinum (CDDP) encapsulated nano liposome (sgc8c-CDDP-NLP) for aptamer (sgc8c) directed therapy of acute lymphocytic leukemia T lymphocytes CCRF-CEM. METHODS Laser nanosize analyzer was used to characterize the scale of sgc8c-CDDP-NLP,UV-VIS-NIR spectrophotometer to detect the encapsulation efficiency of CDDP,isothiocyanate (FITC) to label the nano particles.Fluorescence signal was characterized by fluorescence spectrophotometer and was observed by laser confocal fluorescence microscope in cells. CCK-8 method was used to detect the cell viability. RESULTS The sgc8c-CDDP-NLP was evenly distributed in water,PBS,serum and cell medium for 15 days,and was 115 nm in average diameter,the encapsulation efficiency was 70.9%.Compared with CDDP-NLP,sgc8c-CDDP-NLP efficiently targeted to CCRF-CEM cells instead of NB4 cells and exhibited significant inhibition on CCRF-CEM cells.CONCLUSION The prepreation method used here is feasible and the sample is stable. The sgc8c-CDDP-NLP has great targeting and inhibiting capacity to CCRF-CEM cells.