特发性血小板减少性紫癜患者单个核细胞PD-1/PD-Ls的变化及临床意义 |
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作者姓名: | 周国忠 罗洪强 王慧瑜 |
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作者单位: | 1. 312000 绍兴市人民医院(浙江大学绍兴医院)临床检验中心 |
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基金项目: | 绍兴市科技局一般项目(2012B70051) |
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摘 要: | 目的探讨特发性血小板减少性紫癜(idiopathic thrombocytopenic purpura,ITP)患者外周血程序性死亡因子PD-1及其配体(PD-Ls)的变化及临床意义。
方法收集26例初诊为急性ITP成人患者治疗前、治疗后3个月、6个月以及1年的外周血标本,用流式细胞术检测其外周血T淋巴细胞和单核细胞上分子(CD273、CD274和CD279)的表达水平;对各项指标进行相关性分析和随机区组的方差检验。
结果与ITP患者治疗前相比,治疗后3个月、6个月以及1年的患者单核细胞PD-Ls表达水平均显著性下降(P<0.05);与治疗后3个月相比,治疗后6个月以及1年的患者CD8+T淋巴细胞PD-1表达显著性升高(P<0.05);相关性分析显示ITP患者中单核细胞PD-Ls表达与CD8+T淋巴细胞的PD-1呈正相关(P<0.01)。
结论ITP患者治疗过程中血小板数量的变化与单个核细胞PD-1/PD-Ls的表达水平存在关联性,证实PD-1/PD-Ls可作为观察ITP疗效的动态指标。
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关 键 词: | 特发性血小板减少性紫癜 程序性死亡因子 配体 |
收稿时间: | 2017-11-29 |
Changes and clinical significance of PD-1 and PD-Ls in mononuclear cells in patients with idiopathic thrombocytopenic purpura |
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Authors: | Guozhong Zhou Hongqiang Luo Huiyu Wang |
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Institution: | 1. Shaoxing People′s Hospital (Shaoxing Hospital, Zhejiang University School of Medicime), Shaoxing 312000, China |
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Abstract: | ObjectiveTo investigate the changes and clinical significance of programmed death factor (PD-1) and its ligand (PD-Ls) in peripheral blood mononuclear cells of patients with idiopathic thrombocytopenic purpura (ITP).
MethodsThe expressions of T lymphocytes and monocyte supramolecular molecules (CD273, CD274 and CD279) in peripheral blood of 26 adult patients with acute ITP were detected by flow cytometry before treatment, and 3 months, 6 months and one year after treatment. Correlation of each indice and variance tests of random block was analyzed.
ResultsCompared with ITP patients before treatment, the expression of PD-Ls in monocytes decreased significantly at 3 months, 6 months and one year after treatment (P<0.05), while the expression of PD-1 in CD8+ T lymphocytes increased significantly at 6 months and one year after treatment (P<0.05). Correlation analysis showed that the expression of PD-Ls in monocytes was positively correlated with PD-1 expression in CD8+ T lymphocytes (P<0.01).
ConclusionThe platelet count in patients with ITP were correlated with the expression of PD-1 and PD-Ls in mononuclear cells, which proved that PD-1 and PD-Ls could be used as a dynamic index to observe the therapeutic effect of ITP. |
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Keywords: | Idiopathic thrombocytopenic purpura Programmed death factor-1 Ligand |
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