基于液相色谱-飞行时间质谱法对慈孝竹叶化学成分的快速鉴定

目的：建立高效液相色谱-飞行时间质谱（LC-DAD-ESI-Q-TOF-MS）联用技术快速鉴定慈孝竹叶中的多种化学成分，为慈孝竹叶的开发与利用提供参考。方法：慈孝竹叶甲醇提取物经过Eclipse XD-C₁₈（4.6 mm×150 mm，5 μm）色谱柱，0.1%甲酸水-乙腈洗脱分离后，通过二极管阵列检测器采集紫外数据，最终结合负离子模式下获得的高分辨飞行时间质谱数据对化合物准分子离子峰与特征碎片离子分析，推测慈孝竹叶甲醇提取物的化学成分。结果：在45 min的色谱分离时间内，初步推测出慈孝竹叶中的30种化学成分，其中包括21种黄酮类成分，5种酚酸类成分，2种烯酸类成分，其中，在该植物中首次发现酚酸类、荭草素 4'-O-己糖苷、去甲氧矢车菊黄素7-O-芸香糖苷等12 个化学成分。结论：本研究建立了慈孝竹叶化学成分的快速鉴定方法，可为慈孝竹叶的开发与利用提供基础数据。

Rapid separation and identification of chemical constituents in the leaf of Bambusa multiplex by LC-DAD-ESI-Q-TOF-MS method

OBJECTIVE To establish a rapid method for structural identification of chemical constituents in the leaf of Bambusa multiplex by LC-DAD-ESI-Q-TOF-MS method. METHODS The 50% aqueous-methanol extract of the leaf of Bambusa multiplex was rapidly separated via an Eclipse XD-C₁₈ column (4.6 mm×150 mm,5 μm) and eluted by gradient way with the mobile phase of 0.1% formic acid in water-acetonitrile, and following mass spectra were obtained by ESI-Q-TOF-MS. The obtained data were then analyzed by matching UV spectra and accurate molecular masses of quasimolecular and fragment ions with known chemicals reported in the bamboo leaf. RESULTS Thirty chemicals were tentatively identified in 45 min based on their negative ion ESI MS spectra, including 21 flavonoids, 5 phenolic acids and 2 gadoleic acids. Twelve chemical ingredients were identified for the first time from Bambusa multiplex, such as phenolic acid, orientin 4'-O-hexoside, demethoxycentaureidin 7-O-rutinoside. CONCLUSION A rapid and valid LC-DAD-ESI-Q-TOF-MS method is developed to identify chemicals in the leaf of Bambusa multiplex, which provides meaningful data for development of this herbal medicine.