儿茶素对软脂酸导致的大鼠原代肝细胞损伤的保护作用

目的 探讨儿茶素能否对软脂酸所导致的大鼠原代肝细胞毒性具有保护作用。方法 使用大鼠原代肝细胞分别与毒胡萝卜素和软脂酸以及儿茶素共孵育16 h后检测内质网应激相关蛋白GRP78、GRP94和CHOP,同时检测细胞凋亡和坏死。结果 软脂酸与毒胡萝卜素类似,均可使共孵育的大鼠原代肝细胞GRP78、GRP94和CHOP的水平升高,而儿茶素的加入可以减少细胞凋亡和坏死并显著减少这三种标志物的产生(P＜0.05)。结论 儿茶素可经由抑制内质网应激从而保护细胞不受软脂酸的毒性影响。

Protective effect of catechinic acid against palmitic acid induced endoplasmic reticulum stress-mediated apoptosis in primary rat hepatocytes

Objective To study the ability of catechinic acid to prevent endoplasmic reticulum stress-mediated apoptosis of rat hepatocytes elicited by two compounds,i.e. thapsigargin and palmitic acid. Palmitic acid induced endoplasmic reticulum (ER) stress and caused apoptotic and necrotic cell death in the primary rat hepatocytes.Methods Incubation of primary rat hepatocytes for 16 h with palmitic acid produced a significant increase in cell death. Palmitic acid also increased levels of three indicators of ER stress — glucose regulated protein 78 (GRP78),C/EBP homologous protein (CHOP),and glucose regulated protein 94 (GRP94).Results Catechinic acid distinctly reduced cell death and levels of all three indicators of ER stress brought about by palmitic acid. Thapsigargin,which induces ER stress produced similar effects to those obtained using palmitic acid; its effects were partly reversed by catechinic acid,P＜0.05.Conclusion These results suggest that catechinic acid prevents ER stress-mediated apoptosis of palmitic acid in primary rat hepatocytes.