| 雄土鳖虫活性物质分离工艺考察 |
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| 引用本文： | 王少平,宋晓光,李秉润,孟莹,刘晓美,于帅,刘庆丰,王升光,于盈盈,刘国飞,高鹏,王广录,代龙,杨培民.雄土鳖虫活性物质分离工艺考察[J].中国医院药学杂志,2017,37(21):2135-2138. |
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| 作者姓名： | 王少平 宋晓光 李秉润 孟莹 刘晓美 于帅 刘庆丰 王升光 于盈盈 刘国飞 高鹏 王广录 代龙 杨培民 |
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| 作者单位： | 1. 山东中医药大学, 山东 济南 250355;
2. 安徽生物肽产业研究院有限公司, 山东 芜湖 241000;
3 青岛大学, 山东 青岛 266000 |
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| 基金项目： | 十二五国家科技重大专项重大新药创制课题（编号：2013ZX09103002-021） |
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| 摘 要： | 目的：采用不同方法对雄土鳖虫虫活性物质进行分离，得到活性组分。方法：比较SephadexG15、SephadexG25对样品的分离情况，需用最佳的物质对雄土鳖虫虫活性物质进行分离，然后再采用高效液相仪对分离后的组分进行鉴别，同时采用半制备液相柱对物质进行分离，获得活性单体。并对活性单体进行体外溶栓和抗凝验证。结果：实验得出SephadexG25对雄土鳖虫虫活性物质分离效果比较好。而后采用高效液相仪对分离后的活性组分进行分析，图谱出现五个含量较大的组分。并采用半制备液相柱进行分别收集后，验证得到第二个组分和第三个组分活性较强。结论：采用不同方法对雄土鳖虫虫活性物质进行系统分离后，得到纯度较高的活性组分，为后期研发和应用奠定基础。
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| 关 键 词： | 分离 活性测定 雄土鳖虫 |
| 收稿时间： | 2016-12-27 |
Study on active material separation process of the male Eupolyphaga sinensis Walker |
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| WANG Shao-ping,SONG Xiao-guang,LI Bing-run,MENG Ying,LIU Xiao-mei,YU Shuai,LIU Qing-feng,WANG Sheng-guang,YU Ying-ying,LIU Guo-fei,GAO Peng,WANG Guang-lu,DAI Long,YANG Pei-min.Study on active material separation process of the male Eupolyphaga sinensis Walker[J].Chinese Journal of Hospital Pharmacy,2017,37(21):2135-2138. |
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| Authors: | WANG Shao-ping SONG Xiao-guang LI Bing-run MENG Ying LIU Xiao-mei YU Shuai LIU Qing-feng WANG Sheng-guang YU Ying-ying LIU Guo-fei GAO Peng WANG Guang-lu DAI Long YANG Pei-min |
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| Institution: | 1. Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China;
2. Anhui Biological Peptide Industry Research Institute, Shandong Wuhu 241000, China;
3. Qingdao University, Shandong Qingdao 266000, China |
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| Abstract: | OBJECTIVE To separate active substances of the male Eupolyphaga sinensis Walker by using different methods to get the active components.METHODS The sample was separated by using SephadexG15 and SephadexG25.The best material was used to separate active substances of the male Eupolyphaga sinensis Walker,and the separated components were identified by HPLC.The separation of the substance was carried out by the semi HPLC to get active monomer,which was validated for in vitrothrombolysis and anticoagulation.RESULTS The experiment showed that SephadexG25 was good for the separation of the male Eupolyphaga sinensis Walker.Then the active components were analyzed by HPLC.Five mass fractions were developed and collected by the semi HPLC,respectively.The results showed that the second and third components had the best activity.CONCLUSION Active component of high purity can be obtained using different methods from male Eupolyphaga sinensis Walker,which lays the foundation for further development and application. |
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| Keywords: | separation determination of activity male Eupolyphaga sinensis Walker |
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