一测多评法同时测定姜黄-桂枝药对不同配伍比例中5种有效化学成分含量

目的：建立一测多评法同时测定姜黄-桂枝药对不同配伍比例中肉桂酸、桂皮醛、双去甲氧基姜黄素、去甲氧基姜黄素、姜黄素5种主要成分含量，并验证方法准确性。方法：采用RP-HPLC进行含量测定，色谱柱SHIMADZU VP-ODS C₁₈（250 mm×4.6 mm，5 μm）；流动相为乙腈（A）-0.1%乙酸（B），梯度洗脱；流速1 mL·min^-1；检测波长330 nm；柱温30℃；进样量10 μL。以姜黄素为参照物，建立其对肉桂酸、桂皮醛、双去甲氧基姜黄素、去甲氧基姜黄素的相对校正因子，计算后四者含量，经不同色谱系统和色谱柱间比较验证。同时运用外标法测定姜黄-桂枝药对中5个指标性成分的含量，比较计算值与实测值的差异，以验证一测多评法的准确性和和可行性。结果：在该色谱条件下，桂皮醛、肉桂酸、双去甲氧基姜黄素、去甲氧基姜黄素、姜黄素分别在0.029 7~1.18，0.047 7~1.908，0.016~0.64，0.021~0.84，0.026 4~1.05 6 mg·mL^-1范围内呈现良好的线性关系，加样回收率为99.51%~102.29%，RSD为0.67%~2.60%。该方法具有良好的重复性和耐用性；采用校正因子计算的含量值与外标法实测值之间无显著差异，不同配伍比例下5种有效成分总质量分数以姜黄-桂枝（0：1，1.392%）最高，姜黄-桂枝（1：0，0.293%）最低。结论：一测多评法可用于姜黄-桂枝药对多种成分的质量评价。

Simultaneous determination of five ingredients in Curcumae longa L.-Cassia Twig drug pair by QAMS

OBJECTIVE To establish a method to simultaneously determine contents of cinnamic acid,cinnamaldehyde,bisdemethoxycurcumin,demethoxycurcumin and curcumin in different proportion ratios of Curcumae longa L.-Cassia Twig drug pair.METHODS HPLC method was applied for quality assessment of HPLC analysis performed on a SHIMADZU,VP-ODS C₁₈ (250 mm×4.6 mm,5 μm),with acetonitrile (A)-0.1% acetic acid water solution (B) as the mobile phase,at the flow rate of 1.0 mL·min^-1.An increasing linear gradient of solvent B was used.The detection wavelength was set at 330 nm,the column temperature was maintained at 30℃,and the injection volume was 10 μL.The relative correction factors (RCFs) of curcumin to the other four ingredients were calculated.The method was evaluated by comparison of the quantitative results between external standard method and quantitative analysis of multi-components by single-marker (QAMS) method.RESULTS The peaks of cinnamic acid,cinnamaldehyde,bisdemethoxycurcumin,demethoxycurcumin and curcumin in the sample showed good linear relationship in the ranges of 0.029 7-1.18,0.047 7-0.908,0.016-0.64,0.021-0.84,0.026 4-1.056 μg,respectively.The spiked average recovery was 99.51%-102.29%,RSD 0.67%-2.60%.No significant difference was found in the quantitative results of five ingredients by external standard method or quantitative analysis of multi-components (QAMS) method.Of different proportions ratios of Curcumae Longa L.-Cassia Twig drug pair samples,the ratio (0:1,1.392%) had the highest total contents of the above five ingredients,while (1:0,0.293%) had the lowest.CONCLUSION QAMS method can be used to control the quality of Curcumae longa L-Cassia Twig drug pair.