槲皮素-羟丙基-β-环糊精包合物对柔红霉素心脏毒性的保护作用

目的：研究槲皮素-羟丙基-β-环糊精包合物（QUE-HP-β-CD）对柔红霉素（DNR）心脏毒性的保护作用。方法：将实验小鼠分为6组：正常组每天腹腔注射等量生理盐水；DNR组从实验第9~19天每2日1次腹腔注射3 mg·kg^-1 DNR；包合物不同剂量组于实验第1~17天每日1次分别灌胃50，100，200 mg·kg^-1包合物，并于第9天用与DNR组相同的剂量和方式给予DNR；QUE组用100 mg·kg^-1QUE代替包合物，其余处理方法与包合物组完全相同。结果：QUE和不同剂量包合物均能抑制小鼠体质量降低，但100~200 mg·kg^-1包合物可显著改善DNR所致心肌肥大和心室重构，并使各项心肌酶恢复到正常水平；切片显示100 mg·kg^-1包合物能显著改善DNR所致心肌损伤。结论：QUE-HP-β-CD（100 mg·kg^-1）对抑制DNR所致小鼠心脏毒性效果最好。

Protective effect of quercetin-hydroxypropyl-β-cyclodextrin inclusion complex against daunorubicin-induced cardiotoxicity

OBJECTIVE To investigate the protective effect of quercetin-hydroxypropyl-β-cyclodextrin inclusion complex (QUE-HP-β-CD) against daunorubicin (DNR) -induced cardiotoxicity. METHODS Six groups of mice (n=10) were assigned:control group, equal volume of normal saline was injected intraperitoneally (ip); DNR group, animals received (ip) 3 mg·kg^-1 DNR from day 9 to 19; therapy groups, animals were gavaged (ig) in advance with QUE-HP-β-CD (50, 100, 200 mg·kg^-1, respectively) for 17 days, followed by DNR administration by the same method as that in DNR group; QUE group, QUE-HP-β-CD was replaced by 100 mg·kg^-1 QUE, the other treatments were the same as those in therapy groups. RESULTS Biochemical analysis showed that QUE and every doses of QUE-HP-β-CD inhibited body weight decrease of mice induced by DNR. Moreover, QUE-HP-β-CD (100-200 mg·kg^-1) evidently attenuated myocardial hypertrophy and ventricular remodeling, and reduced the increase of myocardial enzyme levels to return to the normal values. Additionally, histopathological findings exhibited that 100 mg·kg^-1 QUE-HP-β-CD significantly improved the myocardial injury induced by DNR than QUE and other dosage of QUE-HP-β-CD. CONCLUSION QUE-HP-β-CD (100 mg·kg^-1) may have the best protective effect against daunorubicin-induced cardiotoxicity.