MSC介导CD30L等4种炎症相关因子在治疗DMD中发挥作用

目的 研究mUCMSC移植治疗DMD标准模型mdx小鼠后炎症相关因子变化,探讨MSC治疗DMD相关机制。方法 PCR鉴定出mdx小鼠随机分为两组:生理盐水组和治疗组;另取正常C57小鼠作为正常对照组。治疗组腹腔注射mUCMSC的生理盐水细胞悬液,生理盐水组和对照组输入等体积生理盐水,检测血清CK值;对各组小鼠的腓肠肌进行HE染色,显微镜下观察病理变化并计数CNF;采集mdx小鼠腓肠肌组织用QAM-INF-1蛋白芯片测定小鼠肌组织炎症因子含量。结果 治疗组与生理盐水组相比具有差异明显:(1)Mdx小鼠血清CK值生理盐水组为(1374.60±127.13)U/L,治疗组为(434.60±19.39)U/L,显著低于生理盐水组(P<0.05);(2)治疗组mdx小鼠腓肠肌切片显示细胞间炎性细胞浸润减轻,细胞大小趋于统一,核中心移位现象减少;(3)生理盐水组mdx小鼠腓肠肌细胞CNF可达(94.37±11.65)%,治疗组CNF为(80.37±7.65)%,显著减少(P<0.05);(4)mdx小鼠后肢腓肠肌组织中CD30L、IL-21、MIP-1a、PF4含量明显较低(P<0.05)。结论 mUCMSC治疗后,腓肠肌组织中CD30LIL-21、MIP-1a、PF4含量较低,血清CK值降低,细胞损伤减少。肌组织炎症表现减轻,在mUCMSC治疗DMD中发挥作用。

MSC mediates four kinds of inflammation-related factors such asCD30L playing a role in the treatment of DMD

Objective To investigate the effects of inflammation-related factors after mUCMSC transplantation in the treatment of DMD standard model mdx mouse, and to explore some mechanism of MSC treatment of DMD. Methods Mdx mouse identified by PCR were randomly divided into two groups: saline group and treatment group; normal C57 mouse were used as normal control group. The mdx mouse in treatment group received intraperitoneal injection of mUCMSC saline solution, saline solution and control groupreceived an equal volume of normal saline, then tested the serum CK value. The gastrocnemius of each group was stained with HE andpathological changeswere observed under a microscope and the percentages of CNF werecaculated. Thetissue of mdx mouse’s gastrocnemius was used to determine the content of inflammatory factors in mouse muscle tissue by using QAM-INF-1 protein chip.Results There were significant differences in the treatment group compared with the saline group: (1)The serum CK value of Mdx mouse was (1374.60±127.13) U/L, and that of the treatment group was (434.60±19.39) U/L, which was significantly lower than that of the saline group (P<0.05); (2)The gastrocnemius sections of mdx mouse in the treatment group showed a decrease of infiltration of inflammatoryintercellularly. The cell size tends to be uniform and the shift of nuclear centers is reduced;(3)The percentage of CNF of gastrocnemiuscells in saline group was (94.37±11.65)%, and in treatment group was (80.37±7.65)%, which was significantly decreased (P<0.05); (4)The content of CD30L, IL-21, MIP-1a and PF4 in gastrocnemius tissue of mdx mouse was significantly decrease. (P<0.05).Conclusion AftermUCMSC treatment, the serum CK of the treatment group decreased, and the cell damage was reduced. The inflammation of muscle tissue was relieved,with which CD30L,IL-21, MIP-1a, and PF4 were lower, and they played a role in the treatment of DMD by mUCMSC .