木姜子乙醇提取物对肝癌细胞侵袭和迁移能力的影响及机制初探

目的：探讨木姜子乙醇提取物（Litsea pungens ethanol extract，LPEE）对肝癌细胞侵袭转移能力的影响及其相关机制。方法：MTT法测定LPEE对肝癌细胞株HepG2和QGY-7703的IC50；transwell迁移实验和matrigel侵袭实验检测LPEE对HepG2和QGY-7703细胞迁移和侵袭能力的影响；qRT-PCR和Western blot检测LPEE对各组细胞中E-cadherin和VEGF基因和蛋白水平的表达变化。结果：LPEE可显著抑制HepG2和QGY-7703细胞穿过transwell小室和matrigel基质胶的细胞数（P<0.05）；50和100 μg·mL^-1 LPEE作用组与0组相比，可明显降低VEGF mRNA和蛋白水平的表达（P<0.05），而促进E-cadherin mRNA和蛋白水平的表达（P<0.05）。结论：LPEE可抑制肝癌细胞的侵袭和迁移能力，其机制可能与抑制VEGF表达和促进E-cadherin表达有关。

Effects and mechanism of ethanol extract of Litsea pungens on migration and invasion of hepatocellular carcinoma cells

OBJECTIVE To evaluate the effects and mechanism of ethanol extract of Litsea pungens (LPEE) on migration and invasion of hepatocellular carcinoma cells.METHODS IC₅₀ values of LPEE on HepG2 and QGY-7703 were determined by MTT. The migratory and invasive abilities of the HepG2 and QGY-7703 cells were measured by Transwell chamber and Matrigel assays. The mRNA and protein expression of E-cadherin and VEGF were determined in HepG2 and QGY-7703 cells treated with LPEE by qRT-PCR and Western blot.RESULTS LPEE significantly suppressed the migration and invasion of tumor cells into the basement membranes (P<0.05). Compared with 0 group, the groups of 50 μg·mL^-1 and 100 μg·mL^-1 LPEE significantly reduced the expression of mRNA and protein of VEGF (P<0.05), and promoted the expression of E-cadherin (P<0.05).CONCLUSION LPEE can reduce migratory and invasive abilities of hepatocellular carcinoma cells, which may be related to the inhibition of VEGF expression and the promotion of E-cadherin expression.