艾灸血清对体外RA滑膜成纤维细胞增殖的影响

目的 探究艾灸血清对类风湿性关节炎(RA)大鼠滑膜成纤维细胞体外增殖的影响,观察艾灸对实验性RA大鼠的治疗效果。方法 将30只SD大鼠随机分为3组,即模型组、直接灸组、隔姜灸组,每组10只,将每组大鼠造RA模型。造模成功后,模型组不予以任何治疗,直接灸组、隔姜灸法分别给予直接灸法、隔姜灸法刺激RA模型大鼠的“肾俞”、“足三里”。疗程结束后,取腹主动脉血并离心取血清,用含有各组血清培养基培养RA模型滑膜成纤维细胞24 h、36 h、48 h,用CCK8试剂盒检测各组血清培养的滑膜成纤维细胞增殖情况。结果 艾灸血清培养的滑膜成纤维细胞的增殖较模型组血清培养的滑膜成纤维细胞受到抑制,其中36 h隔姜灸组抑制程度最显著。结论 艾灸治疗能显著改善大鼠RA症状,抑制RA滑膜成纤维细胞的增殖,这可能是艾灸发挥抗炎作用的有效机制之一。

Effect of moxibustion serum on the proliferation of rheumatoid arthritis synovial fibroblasts in vitro

Objective To investigate the effect of moxibustion serum on the proliferation of synovial fibroblasts in vitro and to observe the therapeutic effect of moxibustion on experimental RA rats.Methods Thirty SD rats were randomly divided into three groups:model group,direct moxibustion group and Ginger moxibustion group,with 10 rats in each group.RA rats were made in each group.The rats in the model group were not given any treatment after the model established successfully.The direct moxibustion group and the ginger-free moxibustion group were given direct moxibustion and ginger-ginger moxibustion to stimulate“Shensu”and“Zusanli”of RA rats.After the treatment,took the abdominal aorta blood and centrifuged to take the serum,culture medium containing with each group of serum to culture RA synovial fibroblasts for 24 h,36 h,48 h,using CCK8 kit to detect of the synovial fibroblasts proliferation.Results The proliferation of synovial fibroblasts cultured in moxibustion serum was inhibited compared with that in the model group,and the inhibition of ginger moxibustion group was the most significant in the 36-hour.Conclusion Moxibustion treatment can significantly ameliorate the symptoms of RA and inhibit the proliferation of RA synovial fibroblasts,which may be one of the effective mechanisms of moxibustion to achieve anti-inflammatory effects.