三叶青黄酮诱导肺癌SPC-A-1细胞凋亡与cleaved-caspase-3表达的关系

目的：观察三叶青黄酮诱导肺癌SPC-A-1细胞凋亡作用和对cleaved-caspase-3表达的影响，探讨其抗肺癌的可能作用机制。方法：体外培养SPC-A-1细胞，以不同剂量三叶青黄酮进行处理，未给予三叶青黄酮组作为对照组。采用3-（4，5-二甲基噻唑-2）-2，5-二苯基四氮唑溴盐（MTT）比色分析法，观察三叶青黄酮对SPC-A-1细胞增殖抑制作用，计算半数抑制浓度（IC₅₀）；采用流式细胞仪和TUNEL染色检测三叶青黄酮对SPC-A-1细胞凋亡的影响；分别采用免疫细胞分析和蛋白印记分析（Western blot）检测三叶青黄酮对SPC-A-1细胞cleaved-caspase-3表达的影响。结果：三叶青黄酮对SPC-A-1细胞增殖具有明显抑制作用，呈剂量和时间依赖性，并诱导SPC-A-1细胞凋亡，与对照组比较，差异具有统计学意义（P<0.05或P<0.01）；三叶青黄酮显著增加SPC-A-1细胞cleaved-caspase-3表达水平，与对照组比较，差异具有统计学意义（P<0.05或P<0.01）。结论：三叶青黄酮可显著抑制SPC-A-1细胞增殖，并诱导其凋亡，机制可能与增强cleaved-caspase-3表达有关。

Correlation between apoptosis induced by radix tetrastigma hemsleyani flavones and the expression of cleaved-caspase-3 in lung cancer SPC-A-1 cells

OBJECTIVE To observe the effects of radix tetrastigma hemsleyani flavones on induced apoptosis and expression of cleaved-caspase-3 in lung cancer SPC-A-1 cells,and discuss its possible mechanisms of anti-lung cancer.METHODS SPC-A-1 cells were cultured in vitro and treated with radix tetrastigma hemsleyani flavones at various concentrations.Control group without radix tetrastigma hemsleyani flavones was also assigned.The proliferation of SPC-A-1 cells and the 50% inhibitory concentration (IC₅₀) were measured by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) colorimetric assay.The apoptosis of SPC-A-1 cells was observed by flow cytometry and TUNEL staining.The expression of cleaved-caspase-3 was detected by immunocytochemical analysis or western blot analysis.RESULTS Compared with control group,radix tetrastigma hemsleyani flavones inhibited significantly the proliferation of SPC-A-1 cells and induced their apotosis in a dose and time dependent manner (P<0.05 or P<0.01),and also increased significantly the expression and cleaved-caspase-3 in SPC-A-1 cells (P<0.05 or P<0.01). CONCLUSION Radix tetrastigma hemsleyani flavones can inhibit the proliferation of SPC-A-1 cells and induce their apotosis,and its possible mechanisms may be related to enhanced the expression of cleaved-caspase-3.