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反相高效液相色谱法测定人体血浆中维拉佐酮的含量
引用本文:黄洪勇,职晓燕,王传升,杨广声,师天元.反相高效液相色谱法测定人体血浆中维拉佐酮的含量[J].中国医院药学杂志,2017,37(7):596-598.
作者姓名:黄洪勇  职晓燕  王传升  杨广声  师天元
作者单位:1. 新乡医学院第二附属医院国家药物临床试验机构, 河南 新乡 453002; 2. 河南省生物精神病学重点实验室, 河南 新乡 453002
基金项目:河南省卫生厅卫生科技创新型人才工程项目(编号:3052)
摘    要:目的:建立反相高效液相色谱法测定人体血浆中维拉佐酮的含量。方法:色谱柱为美国Diamonsil(250 mm×4.6 mm,5 μm) C18反相柱,流动相为乙腈-甲醇-缓冲液(30∶30∶40,V/V/V),流速为1.0 mL·min-1,柱温30℃;检测波长240 nm。结果:维拉佐酮血浆浓度在0.16~32.00 mg·L-1范围内,线性关系良好(r=0.998 6,n=8),平均日间精密度为4.10%,平均相对回收率为97.85%。结论:本方法准确、快速、简便,适于人血浆中维拉佐酮的血药浓度检测和药动学研究。

关 键 词:维拉佐酮  反相高效液相色谱法  含量  
收稿时间:2016-05-30

Determination of vilazodone in human plasma by RP-HPLC
HUANG Hong-yong,ZHI Xiao-yan,WANG Chuan-sheng,YANG Guang-sheng,SHI Tian-yuan.Determination of vilazodone in human plasma by RP-HPLC[J].Chinese Journal of Hospital Pharmacy,2017,37(7):596-598.
Authors:HUANG Hong-yong  ZHI Xiao-yan  WANG Chuan-sheng  YANG Guang-sheng  SHI Tian-yuan
Institution:1. National Institute for Drug Clinical Trial, Second Affiliated Hospital of Xinxiang Medical University, Henan Xinxiang 453002, China; 2. Henan Provincial Key Laboratory of Biological Psychiatry Resources, Henan Xinxiang 453002, China
Abstract:OBJECTIVE To develop a reverse phase high performance liquid chromatography (RP-HPLC) for the determination of vilazodone in human plasma.METHODS A Diamonsil C18 column (250 mm×4.6 mm, 5 μm) was used with mobile phase composed of acetonitrile-methanol-buffer (30:30:40, V/V/V). Flow rate was 1.0 mL·min-1, column temperature was 30℃. UV detection wavelength was 240 nm.RESULTS In a mass concentration range of 0.16-32.00 mg·L-1, standard curve of vilazodone had a good linearity (r=0.998 6, n=8). The average daytime precision was 4.10%, the average relative recovery was 97.85%.CONCLUSION The established analytical method is accurate, rapid, convenient and suitable for the blood drug concentration detection and pharmacokinetics study of vilazodone in human plasma.
Keywords:vilazodone  RP-HPLC  content  
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