反相高效液相色谱法测定人体血浆中维拉佐酮的含量

目的：建立反相高效液相色谱法测定人体血浆中维拉佐酮的含量。方法：色谱柱为美国Diamonsil（250 mm×4.6 mm，5 μm） C₁₈反相柱，流动相为乙腈-甲醇-缓冲液（30∶30∶40，V/V/V），流速为1.0 mL·min^-1，柱温30℃；检测波长240 nm。结果：维拉佐酮血浆浓度在0.16~32.00 mg·L^-1范围内，线性关系良好（r=0.998 6，n=8），平均日间精密度为4.10%，平均相对回收率为97.85%。结论：本方法准确、快速、简便，适于人血浆中维拉佐酮的血药浓度检测和药动学研究。

Determination of vilazodone in human plasma by RP-HPLC

OBJECTIVE To develop a reverse phase high performance liquid chromatography (RP-HPLC) for the determination of vilazodone in human plasma.METHODS A Diamonsil C₁₈ column (250 mm×4.6 mm, 5 μm) was used with mobile phase composed of acetonitrile-methanol-buffer (30:30:40, V/V/V). Flow rate was 1.0 mL·min^-1, column temperature was 30℃. UV detection wavelength was 240 nm.RESULTS In a mass concentration range of 0.16-32.00 mg·L^-1, standard curve of vilazodone had a good linearity (r=0.998 6, n=8). The average daytime precision was 4.10%, the average relative recovery was 97.85%.CONCLUSION The established analytical method is accurate, rapid, convenient and suitable for the blood drug concentration detection and pharmacokinetics study of vilazodone in human plasma.