黄连联合益生菌改善去卵巢肥胖大鼠左心功能的研究

目的用黄连、二甲双胍、益生菌等对去卵巢肥胖大鼠模型进行干预，观察干预后模型大鼠的代谢指标、左心功能的变化，探讨黄连联合益生菌对去卵巢大鼠的体重、代谢有关指标、左心功能等的作用和可能机制。 方法用40只雌性大鼠，分5组，去双侧卵巢手术后高脂饮食诱导建立去卵巢肥胖大鼠动物模型。分别用二甲双胍、益生菌、灭菌去离子水、黄连联合益生菌灌胃6周（假手术高脂喂养灭菌去离子水灌胃组：缩写为HFSS；去卵巢手术高脂喂养灭菌去离子水灌胃组：缩写为HFOS；去卵巢手术高脂喂养黄连益生菌合剂灌胃组：缩写为HFOH；去卵巢手术高脂喂养二甲双胍灌胃组：缩写为HFOM；去卵巢手术高脂喂养益生菌灌胃组：缩写为HFOB）。测量各组大鼠试验前后的体重，Lee指数；用床边超声心动图测定各组左心功能；处死后测量各组心脏等内脏重量。 结果去卵巢手术术后高脂喂养4周后，去卵巢手术组和假手术组的大鼠体重出现差异（P<0.05）；继续高脂喂养并同时灌胃干预6周后，即术后高脂喂养第10周，各组体重均有明显增加，HFOH组体重增加最慢，HFOM组和HFOB组体重增长较慢，HFOS组体重增长最快；各组大鼠的Lee指数较第1周均有增加，其中最明显的是HFOS组和HFOB组；超声心动图检查显示，HFOH组左心功能指标LVDS和LVESV较HFOS组有明显下降，EF、FS较HFOS组有明显上升；术后高脂喂养第12周处死大鼠称量内脏器官显示，HFOH组的心脏重量最小。 结论黄连、二甲双胍、益生菌对去卵巢高脂饮食诱导肥胖大鼠均有抑制体重快速增加和改善Lee指数增长的作用。其中，黄连联合益生菌干预后，去卵巢肥胖大鼠的左心功能指标改善作用最明显，心脏重量最小。

Study on the improvement of left cardiac function in obese ovariectomized rats with Coptidis rhizoma and probiotics

ObjectiveTo observe the changes of metabolic indexes, left cardiac function of the obese ovariectomized rats after the intervention of Coptidis rhizoma, metformin and probiotics, discuss the possible mechanism of the effects of Coptidis rhizome combined with probiotics on weight, metabolism indexes, left cardiac function of the obese ovariectomized rats. Methods40 female rats were divided into five groups. After bilateral ovariectomy, the animal model of obese ovariectomized rats were induced by high fat diet. Metformin, probiotics, sterilized deionized water and Coptis combined with probiotics were separately gave by gavage for 6 weeks(sham operation with sterilized deionized water gavage group: abbreviation for HFSS; ovariectomized with sterilized deionized water gavage group: abbreviation for HFOS; ovariectomized with Coptidis and probiotics gavage group: abbreviation for HFOH; ovariectomized with metformin gavage group: abbreviation for HFOM; ovariectomized with probiotics gavage group: abbreviation for HFOB). Weighed all rats’ body weight and Lee index before and after the experiment; detected left cardiac function by bedside echocardiography after intervene. After being sacrificed, heart and other organs of each group were weighed. ResultsAfter 4 weeks of high-fat diet, ovariectomized group and sham operation group had a significant difference of weight (P<0.05). Continue the high-fat diet and gavage at the same time for 6 weeks, that’s to say, after 10 weeks of high-fat diet, the weight of all groups increased. HFOH group increased most slowly, HFOM group and HFOB increase slowly and HFOS group is the fastest one. The Lee indexes of five groups increased compared with those of the first week, HFOS group and HFOB group gained the most fast growth. Echocardiography showed that the left cardiac function indexes of HFOH group were significantly lower than other groups such as LVDS and LVESV, the indexes of EF and FS increased significantly compared with other groups. After 12 weeks of high fat feeding, rats were sacrificed to measure their visceral organs which showed heart weight of HFOH group was the lightest. ConclusionCoptidis rhizoma, metformin and probiotics all showed effect of attenuating rapid weight gain and obesity trend in high-fat diet induced obese ovariectomized rats. The intervention of Coptidis rhizoma combined with probiotics showed the most obvious improvement of left cardiac function indexes at the end of experiment and the weight of heart were the minimum.