| 胰蛋白酶酶切EPO之多肽片段抑制EPO促CFU-E形成的研究 |
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| 引用本文： | 黄学文,李素芝,牟信兵,高钰琪,陈有,龙仁玲,黄文超,李旬,孙泽平,洪勇,石泉贵,郭俊,郑健保,潘丽,何贵强,董学文. 胰蛋白酶酶切EPO之多肽片段抑制EPO促CFU-E形成的研究[J]. 西南国防医药, 2007, 17(1): 12-15 |
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| 作者姓名： | 黄学文 李素芝 牟信兵 高钰琪 陈有 龙仁玲 黄文超 李旬 孙泽平 洪勇 石泉贵 郭俊 郑健保 潘丽 何贵强 董学文 |
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| 作者单位： | 1. 第三军医大学高原军事医学系,重庆,400038
2. 西藏军区总医院,西藏,拉萨,850003 |
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| 基金项目： | 拉萨市科技局资助合作项目(2004078) |
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| 摘 要： | 目的:观察胰蛋白酶酶切EPO之多肽片段能否抑制EPO促红系形成。方法:对大鼠骨髓进行红系定向体外培养,加入胰蛋白酶酶切EPO之多肽片段干预,观察红系集落形成单位(CFU-E)之数量,判定胰蛋白酶酶切EPO之多肽片段对红系增殖活性的影响。结果:胰蛋白酶酶切EPO之多肽片段加入干预培养后,CFU-E数量明显减少(P<0.01)。结论:胰蛋白酶酶切EPO之多肽产物体外能对抗EPO,抑制红系增殖。其可作为治疗高原红细胞增多症的可能药物进一步研究。
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| 关 键 词： | 促红细胞生成素 红系集落形成单位 骨髓 |
| 文章编号： | 1004-0188（2007）01-0012-04 |
| 收稿时间： | 2006-10-16 |
| 修稿时间： | 2006-10-16 |
Effects of hydrolysates of EPO on stimulating colony-forming unit-erythropoiesis |
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| HUANG Xue-wen,LI Su-zhi,MU Xin-bin,GAO Yu-qi,CHEN You,LONG Ren-ling,HUANG Wen-chao,LI Xun,SUN Ze-ping,HONG Yong,SHI Quan-gui,QUO Jun,ZHENG Jian-bao,PAN Li,HE Gui-qiang,DONG Xue-wen. Effects of hydrolysates of EPO on stimulating colony-forming unit-erythropoiesis[J]. Medical Journal of National Defending forces in Southwest China, 2007, 17(1): 12-15 |
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| Authors: | HUANG Xue-wen LI Su-zhi MU Xin-bin GAO Yu-qi CHEN You LONG Ren-ling HUANG Wen-chao LI Xun SUN Ze-ping HONG Yong SHI Quan-gui QUO Jun ZHENG Jian-bao PAN Li HE Gui-qiang DONG Xue-wen |
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| Affiliation: | 1. Department of High Altitude Military Medicines, Third Military Medical University, Chongqing, 40038, 2. General Hospital of Tibet Military Command, Lhasa, 850000 |
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| Abstract: | Objective:To observe the effects of the hydrolysates of erythropoietin(EPO) on stimulating colony-forming unit-erythropoiesisin in cultured marrows from rats.Methods:Marrows were collected from rats and the mononuclear cells in the marrows were isolated for erythroid oriented culture. Then the level of CFU-E formation was examined under the conditions treated with EPO or EPO hydrolysates of EPO.Results:It was found that the CFU-E formation was significantly suppressed by the hydrolysates of EPO.Conclusion:The hydrolysates of EPO are able to inhibit the activity of EPO stimulating colony-forming unit-erythroid proliferation in vitro. |
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| Keywords: | erythropoietin colony-forming unit-erythroid marrow |
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