自体免疫性甲状腺疾病中甲状腺组织凋亡与凋亡相关蛋白Fas/FasL和Bcl-2表达研究

目的:探讨细胞凋亡相关蛋白Fas/FasL和Bcl-2在自体免疫性甲状腺疾病(ATDs)中的表达及意义。方法:采用原位细胞凋亡检测(Tunel法)及免疫组化SP法进行检测。桥本甲状腺炎(HT)19例,Graves病(GD)13例,对照组非毒性结节性甲状腺肿(NTNG)(结甲)7例。结果:HT及GD滤泡细胞表达Fas蛋白,阳性率分别为100%和80.01%,结甲组滤泡细胞为57.14%。HT及GD分别与结甲组相比有显著差异(P<0.01)。HT及GD中均有滤泡细胞表达FasL和Bcl-2,而结甲中FasL没有表达。Bcl-2的表达在HT和GD中较强,而在结甲组织中的表达较弱,与HT和GD相比有显著差异(P<0.01)。结论:在HT、GD中滤泡细胞通过表达Fas和FasL,并经过Fas与FaL的作用导致部分滤泡细胞凋亡。在HT、GD中由于滤泡细胞通过增强Bcl-2的表达,从而使得Fas和FasL介导的滤泡细胞凋亡作用减弱。凋亡相关蛋白的表达在自身免疫性甲状腺病的发生发展中有重要作用。

Analysis of apoptosis and expression of apoptosis-related protein Fas/FasL and Bcl-2 in the pathogenesis of autoimmune thyroid disorders

Objective:To investigate the expression of apoptosis-related protein(Fas,FasL,and Bc1-2) in the pathogenesis of autoimmune thyroid disorders(ATDs).Methods:Tunel and immunohistochemical staining was performed on 19 Hashimoto's thyroiditis(HT),13 Graves' disease(GD),and 7 non-toxic nodular goiter(NTNG) as contro1.Results:For Fas positive rates were 100% and 80.01% in HT and GD respectively,positive rate was 57.14% in NTNG,there was a significant difference of Fas positive staining(P<0.01) between HT and NTNG,and between GD and NTNG.HT and GD's follicular cells expressed FasL and Bcl-2,NTNG's follicular cells did not express FasL.Bcl-2 was strongly expressed in HT and GD,in NTNG was weak.there was a significant difference(P<0.01) between HT and GD.Conclusion:The follicular cells in HT and GD undergo apoptosis through expression of Fas/FasL and interaction between them.The down regulation of Bcl-2 expression in thyrocytes can decrease Bcl-2 mediated suppression for apoptosis.Over expression of Bcl-2 could render thyrocytes resistant to Fas/FasL mediated apoptosis.The expression of apoptosis related protein plays an important role in the pathogenesis of ATDs.