两阶段EB病毒酶联免疫吸附法筛查鼻咽癌的探讨

目的：采用两阶段EB病毒血清学筛查方法，进行人群鼻咽癌的筛查。方法：用ELISA检测血清EB病毒抗体，首先以EBNAlIgA作为人群鼻咽癌的初筛指标，第二阶段的检查是在EBNAlIgA阳性人群中再进一步检测EBNAlIgG和ZtaIgG两种抗体。结果：EBNAlIgA的灵敏度和特异度分别为91．9％和91．4％，均高于EBNAlIgG和ZtaIgG；第二阶段检查可以将鼻咽癌筛查的特异度提高到96．5％，同时可将人群划分高、中、低三个不同危险层次，其中高危险人群仅占0．39％。结论：实行两阶段筛查法，使鼻咽癌筛查既能达到良好的灵敏度，又能提高检测的特异性，同时还可以将筛查人群划分不同的危险层次。

Screening out nasopharyngeal carcinoma by two-stage ELISA for EB virus

Objective:Screening out NPC from susceptable crowd by serological test for EB virus.Methods:Checking antibody of EB virus by ELISA. At first, EBNA1 IgA was detected for susceptable crowd as a primary screening index, secondary , EBNA1 IgG and Zta IgG were detected in the EBNA1 IgA positive group.Results:The sensitivity and specificity of EBNA1 IgA were 91.8% and 91.4%, both were higher than those of EBNA1 IgG and Zta IgG; the sencondary detection of EBNA1 IgG and Zta IgG raised the specificity of NPC screening to 96.5% and also helped to divide the crowd into 3 groups as high, median, and low risk. And the high risk group accounted for 0.39%.Conclusion:Two-stage screening of NPC raise the sensitivity and specificity of NPC detection. It also helps to divide the crowd into 3 groups of risk.