Her-2基因表达下调后卵巢癌细胞对化疗敏感性的改变

目的观察Her．2基因表达下调后，卵巢癌细胞系SKOV3．ip1细胞对紫杉醇敏感性的改变。方法1．细胞培养：将原始SKOV3．ip1和低表达Her-2的SKOV3．ip1细胞常规培养于RPM11640培养基，置于37℃，5％CO2培养箱中培养；2-蛋白印迹（WesternBlot）方法检测Her12蛋白表达：WesternBlot方法分别检测Her-2基因在原始SKOV3．ip1和低表达Her-2的SKOV3．ip1细胞的表达，并比较其表达差异；3．3·（4，5-二甲基噻唑-2）-2，5-二苯基四氮唑溴盐（MTT）试验：将不同浓度（0，6，12，24，48，96μg／m1）紫杉醇注射液分别作用于SKOV3．ip1细胞和低表达Her-2的SKOV3．ip1细胞，MTT方法检测细胞抑制率，并比较高、低表达Her-2基因的SKOV3．ip1细胞抑制率的差异。结果在紫杉醇注射液浓度分别为6，12，24，48μg/ml时，低表达Her．2的SKOV3．ip1细胞抑制率分别是（45．50±0．95）％，（65．37±0．56）％，（76．83±0．27）％，（81．83±0．69）％；高表达Her-2的SKOV3．ip1细胞抑制率分别是（36，13±1．74）％，（56．43±2．19）％，（67．57±1．96）％，（74．30±1．10）％。两者比较差异有统计学意义（P〈0．01）；而当紫杉醇注射液浓度达到96μg／ml时，两种细胞的抑制率趋于一致，接近100％。结论Her-2基因表达下调后，SKOV3．ip1细胞对紫杉醇注射液敏感性提高。

The sensitization changes of SKOV3. ip1 cells exposed to taxel when the expression of Her-2 in the cells is depressed

Objective To observe the sensitization changes of SKOV3. ip1 cells exposed to taxel when the expression of Her-2 in SKOV3. ip1 ceils was depressed. Meshods 1. Cell culture: SKOV3. ip1 cells and lower Her-2 expressed SKOV3. ip1 expressed SKOV3. ip1 cells was detected by Western blot. 3. MTT Test : SKOV3. ip1 cells were exposed to different dose taxel (6,12, 24,48,96 μg/ml), The MTT method was used to detect the inhibi-tion rate of SKOV3. ip1 cells exposed to taxel and the inhibition rate of SKOV3. ip1 cells that express different Her-2 level was compared. Results When exposed to(6,12, 24,48 μg/ml)taxel dose, the inhibition rate of lower Her-2 expressed SKOV3. ip1 cells were (45.50±0.95)%, (65.37±0.56)%, (76.83±0.27)% and (81.83±0.69)%. While the inhibition rate of SKOV3. ip1 cells were(36.13±1.74)%, (56.43±2.19)%, (67.57±1.96) % and(74.30±1.10) %. The difference were obviously (P < 0.01). Conclusion The sensitization of SKOV3. ip1 cells to taxel was increased when the expression of Her-2 in the cells was depressed.