骨肉瘤中MMP-2和PCNA表达的意义及相关关系

 目的探讨基质金属蛋白酶-2(MMP-2)、增殖细胞核抗原(PCNA)在骨肉瘤中表达的意义和临床价值.方法采用免疫组织化学s-P法测取32例骨肉瘤、20例骨软骨瘤标本的MMP-2、PCNA表达值,以SPSS10.0软件行数据处理,分析其表达的意义和相关性.结果MMP-2阳性表达率在骨肉瘤为81.25%,在骨软骨瘤为0,两者差异显著(P＜0.01);在骨肉瘤中,MMP-2阳性表达组的软组织浸润或转移比率为73.08%,明显高于阴性表达组(P＜0.05);PcNA的阳性表达率在骨肉瘤中为84.38%,在骨软骨瘤中为10.00%,两者差异显著(P＜0.01);在骨肉瘤中,MMP-2与PCNA呈正相关(rs=+0.703,P＜0.05).结论MMP-2的高表达可能与骨肉瘤浸润转移密切相关,PCNA可作为骨肉瘤增殖的一个标志.

Significance and relationships of expressions of MMP-2 and PCNA in osteosarcoma

Objective To study the significance and clinical value of expressions of matrix metlloproteinase-2(MMP-2) and proliferating cell nuclear antigen(PCNA) in osteosarcoma.Methods MMP-2 and PCNA were detected for the specimens of 32 osteosarcomas and 20 osteochondromas by immunohistochemical S-P method and SPSS 10.0 software was used to analyze the significance and relationships of their expressions.Results The positive rate of MMP-2 was 81.25 percent in osteosarcoma group and zero in osteochondroma group with a significant difference(P<0.01). In osteosarcoma, the rate of invasion or metastasis was 73.08% in MMP-2 positive group,significantly higher than (16.67%) in negatively expressed group (P<0.05). The positive expression rate of PCNA was 84.38% in osteosarcoma group and 10.00% in osteochondroma group,also with a significant difference(P<0.01). In osteosarcoma group, the expression of MMP-2 was positively correlated with the expression of PCNA (r_s= 0.703,P<0.05).Conclusion High expression of MMP-2 is closely correlated with invasion and metastasis of osteosarcoma. PCNA is one of markers of osteosarcoma proliferation.