| 丙型肝炎病毒C基因在E.coli中的高效表达 |
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| 引用本文： | 逯好英 王宇. 丙型肝炎病毒C基因在E.coli中的高效表达[J]. 军事医学科学院院刊, 1998, 22(2): 94-97 |
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| 作者姓名： | 逯好英 王宇 |
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| 作者单位： | [1]军事医学科学院基础医学研究所 [2]北京医科大学肝病研究所 |
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| 基金项目： | 国家“863”高科技资助 |
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| 摘 要： | 目的：通过观察影响ＨＣＶ Ｃ基因在大肠杆菌中表达的因素，研制高表达工程菌株，制备重组抗原，用于免疫诊断和生物学功能研究。
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| 关 键 词： | 丙型肝炎病毒 C基因 基因表达 疏水区编码序列 |
High level expression of HCV nucleocapsid gene in E.coli |
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| Lu Haoying,Jin Ling,Wang Yu,Wang Jie,Zhang Xiaohui,Shang Dan,Ma Xiankai Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing. High level expression of HCV nucleocapsid gene in E.coli[J]. Bulletin of the Academy of Military Medical Sciences, 1998, 22(2): 94-97 |
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| Authors: | Lu Haoying Jin Ling Wang Yu Wang Jie Zhang Xiaohui Shang Dan Ma Xiankai Institute of Basic Medical Sciences Academy of Military Medical Sciences Beijing |
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| Affiliation: | Lu Haoying,Jin Ling,Wang Yu,Wang Jie,Zhang Xiaohui,Shang Dan,Ma Xiankai Institute of Basic Medical Sciences,Academy of Military Medical Sciences,Beijing 100850 |
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| Abstract: | Objective: In order to obtain overexpression product for development of immunodiagnostic assay and study its biological functions, some factors affecting the expression of HCV nucleocapsid gene in E.coli system were observed. Methods: Two gene fragments of the HCV nucleocapsid differing in length were cloned into expression vector with PL promoter. The expressed products were analyzed by SDS PAGE and Western immunoblotting. Results: High level expression was achieved in E.coli system. The expressed product of the gene fragment lacking the hydrophobic carboxyterminus of the HCV nucleocapsid could amount to 38.5% of total bacterial protein in optimum host cells. Conclusion: This study suggests that the gene fragment lacking the hydrophobic carboxyterminus of the HCV nucleocapsid be expressed efficiently in E.coli system. |
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| Keywords: | HCV nucleocapsid protein gene expression hydrophobic fragment coden TAA Escherichia coli |
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