环介导等温扩增快速检测HIV一1DNA病毒

目的建立一种快速、敏感度高的HIV．1DNA病毒检测方法。方法针对HIV一1型病毒序列的特点寻找6个相对保守的区域设计4条环介导等温扩增（LAMP）引物，建立HIV一1DNA—LAMP快速检测方法，并评价其特异性和灵敏度。结果200例HIV感染者外周淋巴细胞中，应用LAMP法检测出195例HIV一1DNA阳性（阳性率为97．5％），50例健康对照结果阴性。采用10倍稀释法，LAMP技术的最低检出限为50copies／m1的HIV一1DNA病毒。对乙型肝炎病毒、疱疹病毒、丙型肝炎病毒进行交叉实验结果均为阴性。结论LAMP是一种快速、敏感度高、特异性强的方法，适合各级医院的临床检测。

Development and evaluation of loop-mediated isothermal amplification for rapid detection of human immunodefi- ciency virus type-1

Objective The objective of this study was to establish a loop-mediated isothermal amplification （LAMP） method for rapid ancl effective detection of human immunodeficiency virus type 1 （ HIV-1 ） DNA. Methods Four LAMP primers specific to six regions of the a conserved part of the HIV-1 genome were designed. We established a novel LAMP method for detecting HIV-1 DNA isolates among 200 HIV-l-infected individuals. Results The LAMP assay detected HIV-1 DNA in peripheral lymphocytes in 195 of 200 samples from 200 HIV-l-infected individuals. All of the control spec- imens showed negative. The detection sensitivity of the method was found to be 50 copies/ml. Using this LAMP assay, the results of cross experimental including Hepatitis B virus, herpes virus, hepatitis C virus, were negative. Conclusion Al- most all HIV-DNA remained in peripheral blood of HIV infection. This LAMP method was convenient, rapid and accurate in detecting HIV-1 DNA. It appeared to be useful for routine clinical practice.