BUB1在鼻咽癌中的表达及临床意义

目的探讨BUB1在鼻咽癌组织中的表达及临床意义。方法应用免疫组化EliVision二步法检测60例鼻咽癌组织、25例慢性鼻咽炎组织标本中BUB1蛋白的表达，并分析其与鼻咽癌患者临床特征的关系。结果鼻咽癌组织中BUBl的阳性表达率为45．0％（27／60），低于慢性鼻咽炎组织的84．0％（21／25），两者差异有统计学意义（P〈0．01）；鼻咽癌颈淋巴结转移组BUB1阳性表达率为30．8％（12／39），低于无淋巴结转移组的71．4％（15／21），两者差异有统计学意义（P〈0．01）；BUB1的表达与鼻咽癌临床分期相关，随临床分期的提高，BUBI的表达逐渐下降，差异有统计学意义（P〈0．01）；BUB1在鼻咽癌中的表达与患者年龄、性别、T分期、颅底破坏及颅神经损伤无相关性（均P〉0．05）。结论BUB1的低表达在鼻咽癌发生、发展中可能发挥重要作用，BUB1可作为鼻咽癌诊断及预后的参考指标之一。

Expression and clinical significance of BUB1 in nasopharyngeal carcinoma

Objective To investigate the expression and clinical significance of BUBI in nasopharyngeal carcinoma （NPC） tissues. Methods EliVision two steps imnmnohistochemical technology was applied to detect the expression of BUB1 protein in 60 cases of NPC tissues and 25 cases of chronic nasopharyngitis tissues, and the relationship of BUB1 ex- pression with clinical features in patients with NPC was investigated. Results The positive rate of BUB1 （45.0% ,27/ 60 ） in NPC tissues was lower than that in chronic nasopharyngitis tissues （84.0% ,21/25 ） ,P 〈 0.01. Tumors with lymph node metastases（30.8%, 12/39） had lower BUBI protein expression than those without metastasis （71.4%, 15/21 ）, P 〈0.01. The positive rate of BUB1 was correlated with clinical stage （ P 〈 0.01 ） , the positive rate of BUB1 was de- creased with clinical stage progress. The expression of BUB1 was not statistically correlated with patient＇ s age, gender,T classification,basilar region destroying and cranial nerve encroaching（P 〉 0.05,respectively）. Conclusion The reduced expression of BUB1 may play a significant role in the tumorigenesis and development of NPC,detection of BUB1 can be a useful marker for diagnosis and prognosis of NPC.