腹腔感染状态下胃粘膜Na+-K+-ATPase活性对胃粘膜电位差的影响

目的 探讨严重腹腔感染状态下大鼠胃粘膜Na^ —K^ —ATPase活性变化对胃粘膜电位差(GTPD)的影响。方法 利用大鼠盲肠结扎穿孔造成腹腔严重感染动物模型，应用电生理记录仪检测穿孔前和穿孔后3、6、12、24、48hGTPD变化；应用生化法测定了各时相大鼠胃粘膜Na^ —K^ —ATPase活性。结果 盲肠穿孔后3hNa^ —K^ —ATPase活性显著降低(P＜0．05)，12h降至最低(P＜0．01)，仅为对照组的48．5％，48h仍未恢复正常。GTPD伤后6h显著下降(P＜0．05)，12h降至最低(P＜0．01)，24h和48h仍显著低于对照组(P＜0．05，P＜0．05)。结论 严重腹腔感染状态下胃粘膜Na^ —K^ —ATPase活性降低可能是引起胃粘膜屏障功能受损的主要原因。

Influence of gastric mucosal Na+-K+-ATPase activity and gastric mucosal transmucosal potential difference during severe intraperitoneal infection in rats

Objective To investigate the effect of gastric mucosal Na + K + ATPase activity on gastric transmucosal potential difference during severe intraperitoineal infection in rats.Methods The intraperitoneal infection rat model was established by cecal ligation and puncture(CLP).The electric physiological recorder meter was used to measure gastric mucosal potential difference.Assay of Na + K + ATPase activity in gastric mucosal tissue was conducted by biochemistry method.Results The activity of Na + K + ATPase was markedly decreased in infected group at 3h after perforation,compared with the control group( P <0.05).There was a minimum of Na + K + ATPase activity at 12h post perforation in infected group( P <0.01),only about 46.6% matched to the control group.Gastric transmucosal potential difference(GTPD)of infected group decreased significantly compared with the control group( P <0.05)at 6h after perforation,and rapidly dropped to the lowest at 12h post perforation( P <0.01).At 24h and 48h after perforation,GTPD was lower in infected group than that in the control group( P <0.05, P <0.05).Conclusion The reduce of Na + K + ATPase activity might be play an important role in gastric mucosal barrier damage following severe abdominal infection induced by CLP.